Comparison of Conjugates Obtained Using DMSO and DMF as Solvents in the Production of Polyclonal Antibodies and ELISA Development: A Case Study on Bisphenol A.

When developing immunochemical test systems, it is necessary to obtain specific antibodies. Their quality depends, among other things, on the immunogen used. When preparing hapten-protein conjugates to obtain antibodies for low-molecular-weight compounds, the key factors are the structure of the hapten itself, the presence of a spacer, the size of the carrier protein and the degree of its modification by hapten molecules.

Dual lateral flow test for simple and rapid simultaneous immunodetection of bisphenol A and dimethyl phthalate, two priority plastic related environmental contaminants.

Contamination of the environment by technogenic endocrine disrupting compounds (EDCs) becomes serious threat to public health. To effectively prevent this threat, it is necessary to improve analytical methods for EDCs to ensure mass, fast and productive monitoring. In the given work, a dual lateral flow test (LFT) is developed in the first time for simultaneous immunodetection of bisphenol A and dimethyl phthalate, priority EDCs releasing from plastic and belonging to different chemical classes.

Sensitive Silver-Enhanced Microplate Apta-Enzyme Assay of Sb Ions in Drinking and Natural Waters.

The toxic effects of antimony pose risks to human health. Therefore, simple analytical techniques for its widescale monitoring in water sources are in demand. In this study, a sensitive microplate apta-enzyme assay for Sb detection was developed.

Development of Lateral Flow Test-System for the Immunoassay of Dibutyl Phthalate in Natural Waters.

The use of a large amount of toxic synthetic materials leads to an increase in the pollution of environmental objects. Phthalates are compounds structurally related to esters of phthalic acid that are widely used in the manufacturing of synthetic packaging materials as plasticizers. Their danger is conditioned by leaching into the environment and penetrating into living organisms with negative consequences and effects on various organs and tissues.

Structure- and Interaction-Based Design of Anti-SARS-CoV-2 Aptamers.

Aptamer selection against novel infections is a complicated and time-consuming approach. Synergy can be achieved by using computational methods together with experimental procedures. This study aims to develop a reliable methodology for a rational aptamer in silico et vitro design.

Raman Scattering-Based Biosensing: New Prospects and Opportunities.

The growing interest in the development of new platforms for the application of Raman spectroscopy techniques in biosensor technologies is driven by the potential of these techniques in identifying chemical compounds, as well as structural and functional features of biomolecules. The effect of Raman scattering is a result of inelastic light scattering processes, which lead to the emission of scattered light with a different frequency associated with molecular vibrations of the identified molecule. Spontaneous Raman scattering is usually weak, resulting in complexities with the separation of weak inelastically scattered light and intense Rayleigh scattering.

Combination of phenylboronic acid and oligocytosine for selective and specific detection of lead(ii) by lateral flow test strip.

Detection of lead (II) in water sources is of high importance for protection from this toxic contaminant. This paper presents the development and approbation of a lateral flow test strip of lead (II) with the use of phenylboronic acid as chelating agent and oligocytosine chain as receptor for the formed complexes. To locate the bound lead (II) on the test strip, phenylboronic acid was conjugated with carrier protein (bovine serum albumin) and applied as a binding line.

Limitations for colorimetric aggregation assay of metal ions and ways of their overcoming.

The development of analytical methods for the determination of metal ions in water is one of the priority tasks for efficient environmental monitoring. The use of modified gold nanoparticles and the colorimetric detection of their aggregation initiated by ions binding with specific receptors on the nanoparticle surface has high potential for simple testing. However, the limits of this approach and the parameters determining the assay sensitivity are not clear, and the possibilities of different assay formats are estimated only empirically.

A highly sensitive label-free amperometric biosensor for norfloxacin detection based on chitosan-yttria nanocomposite.

Here, non-invasive and label-free detection of trace-level of norfloxacin (NF) in human urine samples has been reported using the electrochemical technique. Nanostructured yttrium oxide (nYO) was synthesized at low-temperature using a one-step hydrothermal process. These nYO were characterized by various methods including XRD, FT-IR, Raman spectroscopy, and TEM.

Rapid Visual Detection of Lead and Mercury via Enhanced Crosslinking Aggregation of Aptamer-Labeled Gold Nanoparticles.

A homogenous method is proposed for rapid detection of Pb and Hg using gold nanoparticles conjugated with T,G-rich aptamer. In presence of target ions the modified gold nanoparticles aggregate resulting in change of the solution colour from pink (for scattered nanoparticles) to blue (for their aggregates). Besides, the amplification system for this assay was proposed.

Progress in rapid optical assays for heavy metal ions based on the use of nanoparticles and receptor molecules.

This review (with 230 refs.) covers recent progress in rapid optical assays for heavy metals (primarily lead and mercury as the most relevant) based on the use of nanoparticles and receptor molecules. An introduction surveys the importance, regulatory demands (such as maximum permissible concentrations) and potential and limitations of various existing methods.

ELISA and Lateral Flow Immunoassay for the Detection of Food Colorants: State of the Art.

Food colorants are inalienable part of human life. Since ancient times, they have become firmly established and have undergone a number of changes. During this time, the attitude towards them has changed.

Development of Immunochromatographic Assay for Determination of Tetracycline in Human Serum.

Determining antibiotic concentration in human blood provides useful pharmacokinetic information. Commonly used methods such as ELISA require a long time to obtain results and thus cannot be applied when information is needed immediately. In this study, a novel antibody-based lateral flow technique was developed for tetracycline detection in human serum.

Adsorption of proteins on gold nanoparticles: One or more layers?

Adsorption of proteins on nanoparticles is a complex and poorly studied process. The mechanisms of protein layer formation can fundamentally differ depending on the composition of the medium, the nanoparticles' structure, the protein's nature, and other factors. In particular, monolayer or multilayer immobilization may occur.

Quantum-dot-based immunochromatographic assay for total IgE in human serum.

To rapidly quantify total immunoglobulin E levels in human serum, we developed a novel quantum-dot-based immunochromatographic assay that employs digital recording of fluorescence. It can detect IgE levels of 5-1000 kU/L, with a coefficient of variation ranging from 2.0 to 9.

Quantum dot-based lateral flow immunoassay for detection of chloramphenicol in milk.

A novel rapid (20 min) fluorescent lateral flow test for chloramphenicol (CAP) detection in milk was developed. The chosen format is a binding-inhibition assay. Water-soluble quantum dots with an emission peak at 625 nm were applied as a label.

Advantages of soybean peroxidase over horseradish peroxidase as the enzyme label in chemiluminescent enzyme-linked immunosorbent assay of sulfamethoxypyridazine.

An indirect competitive chemiluminescent enzyme-linked immunosorbent assay (CL-ELISA) of sulfamethoxypyridazine (SMP) was developed. The conjugates of streptavidin with cationic horseradish peroxidase (HRP) and anionic soybean peroxidase (SbP) were used in CL-ELISA for the detection of biotinylated anti-SMP antibodies. For streptavidin-HRP conjugate-catalyzed chemiluminescence measured 20 s after the initiation of the enhanced chemiluminescence reaction (ECR), the limit of detection (IC(10)), the IC(50) value, and the working range in CL-ELISA of SMP are 0.