A targeted UHPLC-MS/MS method to monitor lipidomic changes during a physical effort: Optimization and application to blood microsamples from athletes.

In recent years, lipidomics have been widely developed to try to better understand many diseases or physical conditions. In this study, the aim was to evaluate the possibility to conduct reliable lipidomic studies using hemaPEN® microsampling devices. Targeted lipidomic analysis was applied to investigate the impact of a short and intense physical activity on lipids blood concentration.

Prostate cancer increases hyaluronan in surrounding nonmalignant stroma, and this response is associated with tumor growth and an unfavorable outcome.

Our objective was to investigate whether the presence of a tumor increases hyaluronan (HA) levels in surrounding prostate tissues and whether this extratumoral HA influences tumor growth and outcome. From a series of 287 men diagnosed with prostate cancer at transurethral resection and followed up with watchful waiting, tissue microarrays were constructed, stained, and scored for HA. A high HA staining score in the tumor stroma or in nonmalignant prostate tissue stroma were both associated positively with higher Gleason score and larger tumor volume, and was associated with a poor outcome.