Publications by authors named "Anna Kropiwnicka"
mRNA is a template for protein biosynthesis, and consequently mRNA transport, translation, and turnover are key elements in the overall regulation of gene expression. Along with growing interest in the mechanisms regulating mRNA decay and localization, there is an increasing need for tools enabling convenient fluorescent labeling or affinity tagging of mRNA. We report new mRNA 5' cap analog-based tools that enable site-specific labeling of RNA within the cap using N-hydroxysuccinimide (NHS) chemistry.
View Article and Find Full Text PDFObjectives: The aim of the study was to assess how the morphology of the occlusal surfaces of premolars and molars influenced the occlusion time in a mixed gender group of young adults with full natural dentition.
Methods: Fifty healthy volunteers with Angle's Class I occlusion were included in the study. After clinical examination, the alginate impressions of both arches were taken, and plaster casts were prepared.
Article Synopsis
- The assembly of ribosomes on eukaryotic mRNAs starts with the eIF4E protein attaching to the mRNA 5' end cap, with flowering plants using multiple isoforms of this factor.
- Research focused on five isoforms of eIF4E from Arabidopsis thaliana to analyze their binding affinities to cap analogs, revealing differential strengths in binding.
- Key findings showed that eIF4E binds cap analogs significantly stronger than eIF(iso)4E, while two closely related isoforms, eIF4E1b and eIF4E1c, display different binding efficiencies despite their high sequence similarity.
Aspirin (acetylsalicylic acid, ASA), which is recommended for primary and secondary prevention in diabetes mellitus (DM), has been shown to have a lower antiplatelet activity in diabetic patients. We conducted a crossover designed observational study to evaluate whether there is an association between the parameters relevant to metabolic control of diabetes and platelet sensitivity to aspirin in type 2 diabetic patients. Platelets' ability to adhere and aggregate was monitored with the use of platelet function analyser (PFA-100 collagen/epinephrine closure time, CT(CEPI) or collagen/ADP closure time, CT(CADP)), classical turbidimetric aggregometry and whole blood electrical aggregometry (WBEA), using collagen (WBEA(coll)), ADP (WBEA(ADP)) and arachidonic acid (WBEA(AA)) as platelet agonists, in 48 control healthy volunteers (mean age+/-S.
View Article and Find Full Text PDFEndocrine pancreatic function in patients after acute pancreatitis.
Hepatogastroenterology
March 2003
Background/aims: The incidence of carbohydrate tolerance disorders in patients after acute pancreatitis has not been clearly established yet. The aim of the study was to estimate the frequency of endocrine pancreatic function impairment in patients after acute pancreatitis and its correlation with acute pancreatitis etiology and clinical course.
Methodology: 82 patients (27 women and 55 men), aged between 28-65 (mean: 47 +/- 8.