Ensuring the business continuity of production companies in conditions of COVID-19 pandemic in Poland - Applied measures analysis.

The purpose of the paper is to present the results of the study of solutions implemented in Polish manufacturing companies to ensure their business continuity under COVID-19 pandemic conditions. A questionnaire survey was conducted using the mixed-model CAWI/CATI (Computer Assisted Web Interview/Computer Assisted Telephone Interview) on a representative group of 600 manufacturing companies in Poland. The research was conducted in March 2021, more than a year after the first lockdown.

Cucumber metal tolerance protein CsMTP9 is a plasma membrane H⁺-coupled antiporter involved in the Mn²⁺ and Cd²⁺ efflux from root cells.

Members of the plant metal tolerance protein (MTP) family have been classified into three major groups - Zn-CDF, Mn-CDF and Zn/Fe-CDF - however, the selectivity of most of the MTPs has not been confirmed yet. Cucumber gene CsMTP9 encoding a putative CDF transporter homologous to members of the Mn-CDF cluster is expressed exclusively in roots. The relative abundance of CsMTP9 transcript and protein in roots is significantly increased under Mn excess and Cd.

Two metal-tolerance proteins, MTP1 and MTP4, are involved in Zn homeostasis and Cd sequestration in cucumber cells.

Metal-tolerance proteins (MTPs) are divalent cation transporters that have been shown to be essential for metal homeostasis and tolerance in model plants and hyperaccumulators. Due to the lack of genomic resources, studies on MTPs in cultivated crops are lacking. Here, we present the first functional characterization of genes encoding cucumber proteins homologous to MTP1 and MTP4 transporters.

Molecular and biochemical properties of two P1B2-ATPases, CsHMA3 and CsHMA4, from cucumber.

P1B-ATPases (heavy metal ATPases, HMAs) constitute a multigenic subfamily of P-ATPases involved in the transport of monovalent and divalent heavy metals in plant cells. Here, we present the organization of genes encoding the HMA family in the cucumber genome and report the function and biochemical properties of two cucumber proteins homologous to the HMA2-4-like plant HMAs. Eight genes encoding putative P1B -ATPases were identified in the cucumber genome.