Publications by authors named "Anna Kornmuller"
Article Synopsis
- This study explored how human adipose-derived stromal cells (hASCs) grow on microcarriers made from decellularized adipose tissue (DAT) and decellularized cartilage tissue (DCT) in bioreactor cultures.
- Results showed that hASCs grew denser on DCT microcarriers compared to DAT, and dynamic culture influenced gene and protein expression related to cell adhesion and ECM remodeling.
- Although hASCs showed enhanced lipid accumulation with adipogenic differentiation on both microcarriers, only low levels of chondrogenic differentiation were observed, suggesting that future research should consider other cell types for chondrogenic studies.
Stirred bioreactor systems integrating microcarriers represent a promising approach for therapeutic cell manufacturing. While a variety of microcarriers are commercially available, current options do not integrate the tissue-specific composition of the extracellular matrix (ECM), which can play critical roles in directing cell function. The current study sought to generate microcarriers comprised exclusively of ECM from multiple tissue sources.
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- Researchers created a new 3-D bioscaffold using a "cell-assembly" method composed of decellularized adipose tissue (DAT) beads, aimed at enhancing soft tissue regeneration with high densities of human adipose-derived stromal cells (ASCs).
- In vitro tests showed that the ASCs effectively remodeled the scaffold’s extracellular matrix, maintaining their viability for over a week, especially with growth factor preconditioning that improved scaffold stability.
- In vivo studies indicated that the ASC delivery in the new cell-assembled scaffolds showed better initial cell tracking and enhanced endothelial cell infiltration, suggesting a more stable vascular network compared to traditional methods.
Cell function is mediated by interactions with the extracellular matrix (ECM), which has complex tissue-specific composition and architecture. The focus of this article is on the methods for fabricating ECM-derived porous foams and microcarriers for use as biologically-relevant substrates in advanced 3D in vitro cell culture models or as pro-regenerative scaffolds and cell delivery systems for tissue engineering and regenerative medicine. Using decellularized tissues or purified insoluble collagen as a starting material, the techniques can be applied to synthesize a broad array of tissue-specific bioscaffolds with customizable geometries.
View Article and Find Full Text PDFWith the goal of designing a clinically-relevant expansion strategy for human adipose-derived stem/stromal cells (ASCs), methods were developed to synthesize porous microcarriers derived purely from human decellularized adipose tissue (DAT). An electrospraying approach was applied to generate spherical DAT microcarriers with an average diameter of 428 ± 41 μm, which were soft, compliant, and stable in long-term culture without chemical crosslinking. Human ASCs demonstrated enhanced proliferation on the DAT microcarriers relative to commercially-sourced Cultispher-S microcarriers within a spinner culture system over 1 month.
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