Publications by authors named "Anna Koncz"

In the past decade, extracellular vesicles (EVs) have attracted substantial interest in biomedicine. With progress in the field, we have an increasing understanding of cellular responses to EVs. In this Technical Report, we describe the direct nanoinjection of EVs into the cytoplasm of single cells of different cell lines.

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Cardiomyopathies are leading causes of human mortality. Recent data indicate that the cardiomyocyte-derived extracellular vesicles (EVs) released upon cardiac injury are present in circulation. This paper aimed to analyze EVs released under normal and hypoxic conditions by H9c2 (rat), AC16 (human) and HL1 (mouse) cardiac cell lines.

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Article Synopsis
  • High fat diets (HFD) can lead to serious conditions like dyslipidemia, atherosclerosis, and diabetes, but the effects on liver lipid levels haven't been thoroughly studied before.
  • This study aimed to investigate how HFD affects triglycerides, diglycerides, ceramides, and key lipid-related genes in the liver, while also exploring the role of the protein PCSK9 using specially bred mice.
  • The results showed HFD decreased PCSK9 levels while increasing other lipid-related proteins and hepatic triglycerides, suggesting that HFD-induced changes in liver lipid accumulation mimic those seen in PCSK9 deficiency.
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  • Cardiac cell lines (H9C2, AC16, HL-1) were systematically compared to primary cardiomyocytes and mature cardiac tissues to assess their characteristics and limitations in cardiovascular research.
  • Findings revealed that cardiac cell lines exhibited significantly lower expression of cardiac markers and less pronounced cardiac phenotypes compared to primary and mature cells, even after differentiation.
  • The study concludes that the low resemblance of cell lines to mature cardiac tissue limits their usefulness in research, highlighting the need to consider their translational value when selecting models for cardiomyocyte studies.
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Liver plays a central role in elimination of circulating extracellular vesicles (EVs), and it also significantly contributes to EV release. However, the involvement of the different liver cell populations remains unknown. Here, we investigated EV uptake and release both in normolipemia and hyperlipidemia.

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Iron (Fe) is an essential micronutrient for plants. Due to the requirement for Fe of the photosynthetic apparatus, the majority of shoot Fe content is localised in the chloroplasts of mesophyll cells. The reduction-based mechanism has prime importance in the Fe uptake of chloroplasts operated by Ferric Reductase Oxidase 7 (FRO7) in the inner chloroplast envelope membrane.

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Mast cells have been shown to release extracellular vesicles (EVs) in vitro. However, EV-mediated mast cell communication in vivo remains unexplored. Primary mast cells from GFP-transgenic and wild type mice, were grown in the presence or absence of lipopolysaccharide (LPS), and the secreted EVs were separated from the conditioned media.

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C1-inhibitor (C1-INH) is an important regulator of the complement, coagulation, fibrinolytic and contact systems. The quantity of protease/C1-INH complexes in the blood is proportional to the level of the activation of these four cascade-like plasma enzyme systems. Parallel determination of C1-INH-containing activation complexes could be important to understand the regulatory role of C1-INH in diseases such as hereditary angioedema (HAE) due to C1-INH deficiency (C1-INH-HAE).

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The field of extracellular vesicles (EVs) is an exponentially growing segment of biomedical sciences. However, the problems of normalisation and quantification of EV samples have not been completely solved. Currently, EV samples are standardised on the basis of their protein content sometimes combined with determination of the particle number.

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