A new vector system for targeted integration and overexpression of genes in the crop pathogen .

Background: Besides their ability to produce several interesting bioactive secondary metabolites, members of the species complex comprise important pathogens of plants and humans. One of the major obstacles in understanding the biology of this species complex is the lack of efficient molecular tools for genetic manipulation.

Results: To remove this obstacle we here report the development of a reliable system where the vectors are generated through yeast recombinational cloning and inserted into a specific site in through -mediated transformation.