Optical inactivation of a proprioceptor in an insect by non-genetic tools.

Background: The specific role of sensory organs in locomotor pattern generation is traditionally investigated by means of mechanical ablation in arthropods that currently do not allow genetic manipulation. Mechanical ablation is irreversible, and may lead to injury discharges and changes in the structural integrity of the cuticle.

New Method: Here, we present a new method to temporarily or permanently deprive parts of an insect nervous system of sensory feedback from leg proprioceptors by means of blue light application.

Gradients in mechanotransduction of force and body weight in insects.

Posture and walking require support of the body weight, which is thought to be detected by sensory receptors in the legs. Specificity in sensory encoding occurs through the numerical distribution, size and response range of sense organs. We have studied campaniform sensilla, receptors that detect forces as strains in the insect exoskeleton.

Identification of the origin of force-feedback signals influencing motor neurons of the thoraco-coxal joint in an insect.

Force feedback from Campaniform sensilla (CS) on insect limbs helps to adapt motor outputs to environmental conditions, but we are only beginning to reveal the neural control mechanisms that mediate these influences. We studied CS groups that affect control of the thoraco-coxal joint in the stick insect Carausius morosus by applying horizontal and vertical forces to the leg stump. Motor effects of ablation of CS groups were evaluated by recording extracellularly from protractor (ProCx) and retractor (RetCx) nerves.