Effect of Alpha-Lipoic Acid on Rat Ventricles and Atria under LPS-Induced Oxidative Stress.

Alpha-lipoic acid (α-LA) is a disulfide compound and one of the most effective antioxidants. Many studies have indicated positive effects of α-LA in the prevention of pathologic conditions mediated by oxidative stress, such as cardiovascular diseases. However, the therapeutic potential of α-LA for the heart has not been explored with regards to the ventricles and atria.

[Influence of low frequency magnetic field used in magnetotherapy on interleukin 6 (IL-6) contents in rat heart and brain].

Background: The human population is exposed ever more frequently to magnetic fields (MF). This is due to both technological progress and development of the economy as well as to advances made in medical science. That is why the thorough understanding and systematized knowledge about mechanisms by which MF exerts its effects on living organisms play such an important role.

[Influence of ET-1 and ETA receptor blocker (BQ123) on the level of TNF-α in the brain rat].

Unlabelled: Endothelin 1 (ET-1) in addition to the vasoconstriction, also has mitogenic, proinflammatory and proagregation activities. The mediators of inflammatory responses are cytokines, including special role attributed to tumor necrosis factor (TNF-α).

Aim: The aim of this study was to evaluate the effect of ET-1 and its receptor blocker (BQ123) on the level of TNF-α in the brain rat.

BQ123 Stimulates Skeletal Muscle Antioxidant Defense via Nrf2 Activation in LPS-Treated Rats.

Little is understood of skeletal muscle tissue in terms of oxidative stress and inflammation. Endothelin-1 is an endogenous, vasoconstrictive peptide which can induce overproduction of reactive oxygen species and proinflammatory cytokines. The aim of this study was to evaluate whether BQ123, an endothelin-A receptor antagonist, influences the level of TNF-α, IL-6, SOD-1, HO-1, Nrf2 mRNA, and NF-κB subunit RelA/p65 mRNA in the femoral muscle obtained from endotoxemic rats.

ET-1 mediates the release of reactive oxygen species and TNF-α in lung tissue by protein kinase C α and β1.

Background: The aim of this study was to determine the involvement of protein kinase C (PKC) in the ET-1 induced generation of reactive oxygen species and TNF-α in rat lungs.

Methods: Experiments were performed on 6 groups of rats: Group I: saline-treated control; Group II: saline followed by endothelin-1 (ET-1) (3μg/kg); Group III: saline followed by selective PKC αβ1 inhibitor (Gö6976) (2μg/kg); Group IV: Gö6976 (2μg/kg) administered 30min before ET-1 (3μg/kg); Group V: saline followed by the PKC activator phorbol 12-myristate 13-acetate (PMA) (50μg/kg); Group VI: Gö6976 (2μg/kg) administered 30min before PMA (50μg/kg). After 5h, the animals were euthanized and their lungs were isolated for measurements.

The protective effect of lipoic acid on selected cardiovascular diseases caused by age-related oxidative stress.

Oxidative stress is considered to be the primary cause of many cardiovascular diseases, including endothelial dysfunction in atherosclerosis and ischemic heart disease, hypertension, and heart failure. Oxidative stress increases during the aging process, resulting in either increased reactive oxygen species (ROS) production or decreased antioxidant defense. The increase in the incidence of cardiovascular disease is directly related to age.

[The effects of endothelin 1 and an inhibitor of phosphorylation of IκB on the content of free sulfhydryl groups (-SH) and protein in rat kidney].

Unlabelled: Endothelin (ET)-1 is a potent vasoconstrictor. ET-1 an increase in the synthesis of transcription factor NF-κB, which is responsible for the production of reactive oxygen species (ROS), and cytokines.

Aim: The aim of the study was to determine whether blocking NF-κB pathway affects the content of free -SH groups and total protein in rat kidney homogenates.

Anti-oxidative and anti-inflammatory effects of lipoic acid in rat liver.

Introduction: Lipopolysaccharide (LPS) is a key inflammatory component of Gram-negative bacteria, which after entering the systemic circulation contributes to the development of septic hepatic failure.

Aim: The aim of this study was to evaluate the effects of alpha lipoic acid (LA) on oxidative stress parameters and inflammation in endotoxemic rat liver.

Material/methods: Male Wistar rats were divided into 4 groups, each group consisting of 8 animals.

The influence of a nitric oxide synthase inhibitor and endothelin receptor blocker on the free sulfhydryl groups content in lung homogenates.

Introduction: The aim of the study was to assess the influence of the nitric oxide synthase inhibitor L-NAME and the endothelin receptor blocker BQ123 on the free sulfhydryl (-SH) groups content in rat lung homogenates.

Material And Methods: Experiments were performed on Wistar-Kyoto rats divided into the following groups: group I (control) received (i.v.

Nuclear factor κB inhibitor BAY 11-7082 suppresses oxidative stress induced by endothelin-1 (ET-1) in rat kidney.

Aim: The aim of the study was to evaluate the effect of BAY 11-7082, an NF-κB inhibitor, on basal and ET-1-induced production of reactive oxygen species (ROS), TNF-α and p65 protein in rat kidney.

Material/methods: The experimental animals were divided into five groups (n=7) receiving: 1) saline (control); 2 and 3) ET-1 in a dose of 3 μg/kg body weight (b.w.

Estimation of Some Oxidative Stress Parameters and Blood Pressure After Administration of Endothelin-1 (ET-1) in Rats.

The aim of the study was to investigate changes in the plasma antioxidative activity and in lipid peroxidation after administration of endothelin-1 (ET-1) and endothelin receptor blockers and additionally, to estimate blood pressure. The study was performed on male Wistar rats (n = 6 per group) divided into 4 groups which received: (1) saline, (2) endothelin-1 (ET-1) (3 μg/kg b.w.

The role of endothelin-1 and endothelin receptor antagonists in inflammatory response and sepsis.

Endothelin-1 (ET-1) is a potent endogenous vasoconstrictor, mainly secreted by endothelial cells. It acts through two types of receptors: ETA and ETB. Apart from a vasoconstrictive action, ET-1 causes fibrosis of the vascular cells and stimulates production of reactive oxygen species.

[Effect of extremely low frequency magnetic field on glutathione in rat muscles].

Background: Free radicals (FR) are atoms, molecules or their fragments. Their excess leads to the development of oxidizing stress, the cause of many neoplastic, neurodegenerative and inflammatory diseases, and aging of the organism. Industrial pollution, tobacco smoke, ionizing radiation, ultrasound and magnetic field are the major FR exogenous sources.

[Endothelin receptor antagonists--a brief description of the new class of drugs].

Endothelins are endothelial peptides, the properties of which have been investigated for over 25 years. They play a special role in the pathophysiology of many diseases of the cardiovascular system. Endothelin-1, which is the most explored one, is a potent vasoconstrictor.

[Influence of lipoic acid on the level of TNF-alpha in spleen homogenates].

Unlabelled: Lipoic acid (LA) is a natural antioxidant and possess beneficial effects on oxidative stress parameters in various tissues. The aim of the study was to estimate of influence of LA on free -SH groups and TNF-alpha levels in spleen homogenates in rats during oxidative stress induced by LPS.

Material And Methods: Experiments were performed on Wistar rats devided into 4 groups: group I (control) received 0,9% NaCl; group II --received LA (100 mg/kg); group III received LPS (10 mg/kg); group IV--received LPS (10 mg/kg) and 30 min later received LA at the dose 100 mg/kg.

[Influence of extremely low frequency magnetic field on total protein and -sh groups concentrations in liver homogenates].

Background: Free radicals are atoms, molecules or their fragments, whose excess leads to the development of oxidative stress, the cause of many neoplastic, neurodegenerative and inflammatory diseases, as well as aging of organisms. Industrial pollution, tobacco smoke, ionizing radiation, ultrasound and magnetic fields are the major exogenous sources of free radicals. The low frequency mag- netic field is commonly applied in physiotherapy.

Influence of NADPH oxidase inhibition on oxidative stress parameters in rat hearts.

Background: The aim of this study was to assess whether apocynin, an nicotinamide adenine dinucleotide phosphate (NADPH) oxidase blocker, influences lipid peroxidation TBARS, hydrogen peroxide (H2O2) content, protein level, heart edema, tumor necrosis factor α (TNF-α) concentration or the glutathione redox system in heart homogenates obtained from endothelin 1 (ET-1)-induced oxidative stress rats.

Methods: Experiments were carried out on adult male Wistar-Kyoto rats. The animals were divided into 4 groups: Group I: saline-treated control; Group II: saline followed by ET-1 (3 μg/kg b.

The influence of chromone based hydrazones on lipid peroxidation and bFGF concentration in the HL-60 cell line.

Natural and synthetic derivatives of benzo-γ-pyrones (i.e. flavones, chromones, and coumarins) and their synthetic analogues possess a wide range of biological properties in vitro and in vivo.

[Influence of nuclear factor-kappa B inhibition on -SH groups concentration in liver of rats].

The Aim Of The Study: was to evaluate the influence of nuclear factor-kappaB inhibition on -SH groups concentration in liver of rat

Material And Methods: Experiments were performed on Wistar-Kyoto rats divided into following groups: group I (control) received saline; group II (ET-1) received endothelin 1 (12.5 microg/kg b.w.

Influence of block of NF-kappa B signaling pathway on oxidative stress in the liver homogenates.

The aim of the present study was to assess whether BAY 11-7082, a nuclear factor-kappaB (NF- κ B) inhibitor, influences the level of reactive oxygen species (ROS), tumor necrosis factor alpha (TNF- α), and NF- κ B related signaling pathways in the liver. The animals were divided into 4 groups: I: saline; II: saline + endothelin-1 (ET-1) (1.25 μg/kg b.

Effects of lipoic acid on spleen oxidative stress after LPS administration.

Background: Bacterial endotoxin (lipopolysaccharide - LPS) is a strong modulator of the immune system that plays a crucial role in the pathogenesis of endotoxic shock. The aim of the study was to investigate the effects of lipoic acid (LA) on oxidative stress markers in spleen homogenates obtained from LPS-induced endotoxic shock rats.

Methods: The animals were treated with saline or lipoic acid (LA) (60 or 100 mg/kg b.

Influence of specific endothelin-1 receptor blockers on hemodynamic parameters and antioxidant status of plasma in LPS-induced endotoxemia.

Background: The potent vasoconstrictor endothelin-1 has been implicated in the pathogenesis of plasma oxidative stress seen in sepsis. The selective endothelin receptor blockers BQ123 and BQ788 were used to investigate the importance of selective endothelin receptor blockage in modulating oxidative stress during endotoxemia.

Methods: The study was performed on male Wistar rats (n = 6 per group) divided into groups: (1) saline, (2) lipopolysaccharide (LPS) (15 mg/kg)-saline, (3) BQ123 (0.

The role of endothelin-1 and its receptor blockers on the liver function.

Endothelin-1 (ET-1) was first described by Yanagisawa et al. (1988) as a 21-amino acid peptide present in the extract from the aorta endothelial cells. It is known that ET-1 is one of the most potent vasoconstrictor compounds and also causes proliferation of many of the vascular cells involved in vascular remodeling.

The influence of ETA and ETB receptor blockers on LPS-induced oxidative stress and NF-κB signaling pathway in heart.

The aim of this study was to assess whether an endothelin-A receptor (ETA-R) blocker, BQ123, or an endothelin-B (ETB-R) receptor blocker, BQ788, influences nuclear factor kappa beta (NF-κB) pathway, free radical generation, tumor necrosis factor-alpha (TNF-α) concentration, and glutathione redox system in hearts obtained from lipopolysaccharide (LPS)-induced endotoxic rats. The study was performed on rats divided into groups: 1) saline, 2) saline + LPS (15 mg/kg), 3) BQ123 (1 mg/kg b.w.