Formation of the Francisella tularensis Biofilm is Affected by Cell Surface Glycosylation, Growth Medium, and a Glucan Exopolysaccharide.

Biofilms are matrix-associated communities that enable bacteria to colonise environments unsuitable for free-living bacteria. The facultative intracellular pathogen Francisella tularensis can persist in water, amoebae, and arthropods, as well as within mammalian macrophages. F.

Corrigendum: Glycosylation of a Capsule-Like Complex (CLC) by Is Required for Virulence and Partial Protective Immunity in Mice.

[This corrects the article DOI: 10.3389/fmicb.2017.

Further Characterization of the Capsule-Like Complex (CLC) Produced by Subspecies : Protective Efficacy and Similarity to Outer Membrane Vesicles.

is the etiologic agent of tularemia, and subspecies (type A) is the most virulent subspecies. The live vaccine strain (LVS) of subspecies produces a capsule-like complex (CLC) that consists of a large variety of glycoproteins. Expression of the CLC is greatly enhanced when the bacteria are subcultured in and grown on chemically defined medium.

Glycosylation of a Capsule-Like Complex (CLC) by Is Required for Virulence and Partial Protective Immunity in Mice.

is a Gram-negative bacterium and the etiologic agent of tularemia. may appear encapsulated when examined by transmission electron microscopy (TEM), which is due to production of an extracellular capsule-like complex (CLC) when the bacterium is grown under specific environmental conditions. Deletion of two glycosylation genes in the live vaccine strain (LVS) results in loss of apparent CLC and attenuation of LVS in mice.

Prevalence and characterization of methicillin-resistant Staphylococcus aureus isolates from healthy university student athletes.

Background: The prevalence of methicillin-resistant Staphylococcus aureus (MRSA) has been increasing in the general population, and there is concern that close or physical contact, such as in professional and collegiate sports, may increase spread of MRSA. We sought to determine the prevalence of MRSA colonization of male and female athletes from 9 different sports at a major, Division I University during a 12-week period, and determine the USA and SCCmec type from select isolates.

Methods: Swabs for culture of MRSA were obtained from nasal, axillary, and inguinal sites from healthy, asymptomatic student athletes and support staff each week for 12 weeks.

Isolation and mutagenesis of a capsule-like complex (CLC) from Francisella tularensis, and contribution of the CLC to F. tularensis virulence in mice.

Background: Francisella tularensis is a category-A select agent and is responsible for tularemia in humans and animals. The surface components of F. tularensis that contribute to virulence are not well characterized.