Ann Ist Super Sanita
February 2017
Introduction: The study aims to investigate the change in the burden of disease and the microbiological characteristics of Invasive Meningococcal Disease (IMD) in Puglia in comparison with overall incidence from 1994 through 2014.
Methods: Data are gathered in the frame of the National Surveillance System coordinated by the National Reference Laboratory (NRL) of the Istituto Superiore di Sanità.
Results: In Puglia, from 1994 through 2014, the average incidence of IMD was 0.
The lack of rapidity and the low sensitivity and specificity of traditional laboratory methods limits their usefulness in the laboratory diagnosis of viral central nervous system (CNS) infections. This study describes the use of a commercially available multiplex polymerase chain reaction (mPCR)-based reverse hybridization assay (RHA) for the simultaneous detection of the genomes of 8 viruses and Toxoplasma gondii in cerebrospinal fluids (CSF) from 181 patients suspected of having viral meningitis. Twenty-two/181 (12.
View Article and Find Full Text PDFBackground: A limited number of human papillomavirus (HPV) types account for the majority of invasive cervical cancer cases.
Objectives: To assess, in a southern Italian region, where HPV infection had not yet been investigated, the prevalence of type-specific HPV infection.
Study Design: Multiplex PCR was used to test cervical specimens from 871 asymptomatic women.
Infection is a serious complication following pacemaker implantation. Usually it results from normal flora of the skin or from Enterobacteriaceae, Pseudomonas aeruginosa, Streptococcus spp., Enterococcus spp.
View Article and Find Full Text PDFBDProbeTec ET (Becton Dickinson, Sparks, Md, USA) is a fully automated walkaway system based on strand displacement amplification (SDA) technology that provides a method for the direct detection of Mycobacterium tuberculosis complex (MTBC) target sequence. The purpose of this study was to evaluate the ability of BDProbeTec ET system to detect MTBC directly from clinical specimens and compare the results with staining and culture. From February 2002 through December 2003 a total of 1521 [pulmonary (n=1329) and extrapulmonary (n=192)] specimens from 1518 patients were examined by BDProbeTec ET system for the detection of MTBC and the results were compared to those obtained by microscopy and liquid culture (BACTEC 9000 MB, Becton Dickinson).
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