Effect of uremia and hemodialysis on proteome profile of blood platelets and plasma.

The aim of the present research was to assess the differences in blood platelet and plasma proteome profiles of patients with uremia in comparison with healthy participants. It was found that 23 peptides in the platelet proteome profiles of hemodialyzed patients and only 6 peptides in nondialyzed patients were upregulated. On the other hand, 18 peptides with reduced expression in nondialyzed patients and only 1 peptide in hemodialyzed patients were found.

Effect of uremia and hemodialysis on platelet apoptosis.

The blood platelet proteome of hemodialyzed patients with uremia exhibits significant difference in comparison with the blood platelet proteome of healthy individuals. This alteration is manifested by the presence of high concentrations of low-molecular peptides within the whole range of isoelectric points. Increased platelet apoptosis has been put forward as a possible cause of this phenomenon.

Fractions of total plasma homocysteine in patients with ischemic stroke before the age of 55 years.

The mechanism responsible for the association between elevated circulating homocysteine levels and ischemic stroke remains unclear. Therefore, the authors assessed total plasma homocysteine (tHcy) and its fractions (free [fHcy] and protein-bound [bHcy] homocysteine) in patients with ischemic stroke before the age of 55 years. Fifty patients (23 men, mean age 46.