Publications by authors named "Anna B Kolasa"
In recent years, technologies capable of analyzing single cells have emerged that are transforming many fields of biological research. Herein we report how DNA-functionalized hydrogel beads can serve as a matrix to capture mRNA from lysed single cells. mRNA quantification free of pre-amplification bias is ensured by using padlock probes and rolling circle amplification followed by hybridization with fluorescent probes.
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- Cell-penetrating peptides (CPPs) can transport therapeutic agents across cell membranes, but their entry mechanisms are not fully understood.
- This study specifically investigates the membrane permeability sequence (MPS) peptide linked to 5' mRNA cap analogues, finding that the MPS peptide can translocate across membranes of giant unilamellar vesicles (GUVs).
- The research demonstrates that while the MPS peptide itself can cross membranes, passive translocation does not occur for its conjugates, although some permeability was seen with mononucleotide analogues, emphasizing the importance of using artificial membranes for studying these mechanisms.