Publications by authors named "Ann-Sofie Rytting"
Drug resistance is becoming a problem in the treatment of the human immunodeficiency virus type one (HIV-1). To obtain therapeutic DNA vaccines that would target multiple drug-resistance (DR) mutations, we cloned genes for DR HIV-1 reverse transcriptase (RT) and codon-optimized synthetic genes encoding clusters of human CTL epitopes located at the sites of DR-mutations (RT minigenes) and antibody and CTL-epitope tags. Expression of RT genes/minigenes in eukaryotic cells was confirmed by Western blotting and immunofluoresence staining with RT- or tag-specific antibodies.
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- Mutations in reverse transcriptase (RT) can make HIV more resistant to drugs but may reduce the virus's ability to replicate.
- The study aimed to investigate how these drug-resistance mutations affect the expression of HIV RT in different cell types.
- Findings showed that as more drug-resistance mutations accumulated, RT expression levels decreased due to instability caused by potential cleavage sites near the mutations, which may contribute to the lower replication capacity of drug-resistant HIV strains.