Immobilization of β-Galactosidase From Aspergillus oryzae on Electrospun Gelatin Nanofiber Mats for the Production of Galactooligosaccharides.

Two simple and easily reproducible methods for the immobilization of β-galactosidase (β-gal) from Aspergillus oryzae on electrospun gelatin nanofiber mats (GFM) were developed. The process was optimized regarding the electrospinning solvent system and the subsequent cross-linking of GFM in order to increase their stability in water. β-Gal was covalently immobilized on activated gelatin nanofiber mats with hexamethylenediamine (HMDA) as a bifunctional linker and secondly via entrapment into the gelatin nanofibers during the electrospinning process (suspension electrospinning).

Chitosan-based hybrid immobilization in bienzymatic reactions and its application to the production of laminaribiose.

A hybrid-immobilization method was developed to improve the long-term stability of laminaribiose phosphorylase immobilized on epoxy supports Sepabeads EC-EP/S. Entrapment in chitosan retained all of the enzyme activity depending on the amount of entrapped solid materials and increased half-life by a factor of 10-94.4 h.