Publications by authors named "Ann-Catherine Laliberte"
Article Synopsis
- Plant molecular farming is shifting from soil-based setups to hydroponic systems, with this study focusing on optimizing H1 protein expression in Nicotiana benthamiana using nutrient film technique (NFT).
- High plant density and the addition of the cytokinin 6-BAP negatively impacted both axillary leaf biomass and H1 yield per plant, showing a 30% drop in leaf biomass and a 39% decrease in H1 yield per plant.
- Doubling the daily light integral (DLI) led to a significant increase in H1 yield, with the upper leaves on the main stem contributing about 80% to the total yield, highlighting different growth and response patterns in NFT systems compared to traditional potted plants.
Data are scarce about the influence of basic cultural conditions on growth patterns and overall performance of plants used as heterologous production hosts for protein pharmaceuticals. Higher plants are complex organisms with young, mature, and senescing organs that show distinct metabolic backgrounds and differ in their ability to sustain foreign protein expression and accumulation. Here, we used the transient protein expression host as a model to map the accumulation profile of influenza virus hemagglutinin H1, a clinically promising vaccine antigen, at the whole plant scale.
View Article and Find Full Text PDF