A total maximum daily load for the Chesapeake Bay requires reduction in pollutant load from sources within the Bay watersheds. The Conestoga River watershed has been identified as a major source of sediment load to the Bay. Upland loads of sediment from agriculture are a concern; however, a large proportion of the sediment load in the Conestoga River has been linked to scour of legacy sediment associated with historic millpond sites.
View Article and Find Full Text PDFRestoration and reconnection of floodplain systems provide multiple societal and ecosystem benefits, while providing municipalities the opportunity to attempt alternative approaches to maintain infrastructure protection and function. In some restored floodplains, treated wastewater effluent discharge is redirected over land instead of directly into rivers to allow natural flow and infiltration, to facilitate restoration designs such as levee setback, and to provide additional freshwater to floodplain ecosystems. However, indirect discharge of treated effluent over land may pose risks to surface and groundwater when pollutants like excess nutrients enter the floodplain and undergo transformation.
View Article and Find Full Text PDFHyporheic exchange between a river channel and its floodplain region assists in mediating processes such as nutrient removal and temperature regulation. Floodplain restoration in the form of levee setbacks are often carried out to improve the hyporheic exchange. In this study Light Detection and Ranging (LiDAR) data were used along with the head data from observation wells and stage data from rivers to setup and calibrate a groundwater model for 458 km of area within Gap to Gap reach of the Yakima River, WA.
View Article and Find Full Text PDFEnviron Sci Technol
August 2013
qPCR methods are able to analyze DNA from microbes within hours of collecting water samples, providing the promptest notification and public awareness possible when unsafe pathogenic levels are reached. Health risk, however, may be overestimated by the presence of extracellular DNA (eDNA) that is corecovered by the filtration procedure which is the most commonly used method to concentrate target microbes from environmental waters. Using C.
View Article and Find Full Text PDFPurified oocysts of Cryptosporidium parvum were used to evaluate the applicability of two quantitative PCR (qPCR) viability detection methods in raw surface water and disinfection treated water. Propidium monoazide-qPCR targeting hsp70 gene was compared to reverse transcription (RT)-qPCR heat induced hsp70 mRNA in water samples spiked with oocysts. Changes in viability of flow cytometry sorted fresh and oocysts having undergone various aging periods (up to 48 months at 4 °C) were evaluated by Ct values obtained from the qPCR before and after disinfection scenarios involving ammonia or hydrogen peroxide.
View Article and Find Full Text PDFMental health nursing faculty are challenged to connect with students on an affective level in their courses. One important strategy to promote affective learning is to understand the experience of the family caregiver. There is relatively little student access to family caregivers of individuals who are identified as severely and persistently mentally ill.
View Article and Find Full Text PDFAppl Environ Microbiol
September 2011
Extraction of high-quality mRNA from Cryptosporidium parvum is a key step in PCR detection of viable oocysts in environmental samples. Current methods for monitoring oocysts are limited to water samples; therefore, the goal of this study was to develop a rapid and sensitive procedure for Cryptosporidium detection in soil samples. The efficiencies of five RNA extraction methods were compared (mRNA extraction with the Dynabeads mRNA Direct kit after chemical and physical sample treatments, and total RNA extraction methods using the FastRNA Pro Soil-Direct, PowerSoil Total RNA, E.
View Article and Find Full Text PDFPurpose: To explain how baccalaureate-prepared acute care nurses understand, adapt to, and negotiate challenge and change in acute care settings in the context of social and structural features and consequently develop career persistence there.
Design: Grounded theory method based on the research of Strauss and Corbin.
Methods: A research team conducted open-ended interviews with a theoretic sample of 19 new and experienced baccalaureate-prepared (BSN) nurses in the southeast United States during 2007 and 2008 until saturation was achieved.
Nurs Educ Perspect
June 2008
New nurses typically begin their practice in acute care settings in hospitals, where their work is characterized by time constraints, high safety risks for patients, and layers of complexity and difficult problems. Retention of experienced nurses is an issue central to patient safety. The purpose of this qualitative study was to explore the nature of professional resilience in new baccalaureate-prepared nurses in acute care settings and to extrapolate pedagogical strategies that can be developed to support resilience and career longevity.
View Article and Find Full Text PDFRelative changes in the microbial quality of Lake Texoma, on the border of Texas and Oklahoma, were investigated by monitoring protozoan pathogens, fecal indicators, and factors influencing the intensity of the microbiological contamination of surface water reservoirs. The watershed serves rural agricultural communities active in cattle ranching, recreation, and is a potential drinking water source. A total of 193 surface water samples were tested over a 27-month period to determine levels of parasite contamination.
View Article and Find Full Text PDFA field study was conducted to evaluate the performance of a ferrous iron based in situ redox zone for the treatment of a dissolved phase Cr(VI) plume at a former industrial site. The ferrous iron based in situ redox zone was created by injecting a blend of 0.2 M ferrous sulfate and 0.
View Article and Find Full Text PDFNew nurses seem unable to find a means of flourishing professionally in acute care practice and, consequently, exit far earlier than expected. Worldviews of baccalaureate students have changed from previous generations; yet, the approaches to nursing education remain essentially the same. Just as clinical settings benefit from nursing theory as the basis for nursing practice and scientific inquiry, the science of nursing education would benefit from nursing theory as a basis for guiding educational practice.
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