Isolation and Characterization of in Bovine Milk from Rewa, India.

Mastitis in dairy animals affect milk quality and quantity, animal health and welfare, antimicrobial use and economics of dairy farm, and antimicrobial residues in milk. is most common mastitis pathogen with ability to cause infections which are difficult to treat. The present study aimed to characterize the strains associated with dairy animal with reference to its virulence, biofilm formation, antimicrobial resistance including methicillin and penicillin G resistance.

Development and evaluation of probe based real time loop mediated isothermal amplification for Salmonella: A new tool for DNA quantification.

A one step, single tube, accelerated probe based real time loop mediated isothermal amplification (RT LAMP) assay was developed for detecting the invasion gene (InvA) of Salmonella. The probe based RT LAMP is a novel method of gene amplification that amplifies nucleic acid with high specificity and rapidity under isothermal conditions with a set of six primers. The whole procedure is very simple and rapid, and amplification can be obtained in 20min.