Publications by authors named "Ankur Singh"

The Ras GTPase-activating protein SH3-domain-binding protein 1 (G3BP1) serves as a formidable barrier to viral replication by generating stress granules (SGs) in response to viral infections. Interestingly, viruses, including SARS-CoV-2, have evolved defensive mechanisms to hijack SG proteins like G3BP1 for the dissipation of SGs that lead to the evasion of the host's immune responses. Previous research has demonstrated that the interaction between the NTF2-like domain of G3BP1 (G3BP1) and the intrinsically disordered N-terminal domain (NTD-N) of the N-protein plays a crucial role in regulating viral replication and pathogenicity.

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  • Desmodesmus pannonicus IITISM-DIX2 was found to be more effective than Chlorella sorokiniana IITISM-DIX3 in breaking down caffeine, leading to the creation of a predictive artificial neural network (ANN) model for caffeine removal efficiency.
  • The ANN model, structured with a 4-15-1 multilayer perceptron, exhibited high accuracy with a correlation coefficient (R) over 0.96, showing that pH was the most important variable impacting caffeine degradation.
  • Indole acetic acid (IAA) not only improved lipid content in Desmodesmus by 91% in caffeine-laden wastewater but also positively influenced metabolic pathways, highlighting its potential in enhancing caffeine removal
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DNA's extraordinary potential reaches far beyond its role as a carrier of genetic information. It serves as a remarkably adaptable structural foundation for constructing intricate nanostructures with a diverse range of functionalities. This inherent programmability sets DNA apart from other biomolecules like peptides, proteins, and small molecules.

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B cell maturation depends on cognate interactions between the T and B cells. Upon interaction with CD40 ligand (CD40L) on T cells, CD40 delivers costimulatory signals alongside B cell antigen receptor (BCR) signaling to regulate affinity maturation and antibody class switch. Mutations affecting CD40-CD40L interactions cause abnormal antibody responses in immunodeficiencies known as X-linked hyper-IgM syndrome (X-HIgM).

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Objective: To identify the most commonly reviewed behaviour change techniques (BCTs) and their effectiveness based on consistency across reviews for lifestyle interventions of non-communicable diseases.

Design: Umbrella review of systematic reviews.

Data Sources: PubMed, Embase, PsycINFO, Cochrane CENTRAL, Global Health.

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  • - Antibodies are generated by naive B cells turning into plasma cells in germinal centers (GCs) of lymphoid tissues, and patients with B cell lymphoma undergoing immunotherapy have reduced antibody production, leading to higher infection rates and weaker vaccine responses.
  • - Current research models struggle to effectively mimic long-term GC functions and assess B cell responses, prompting the development of synthetic hydrogels that replicate the lymphoid tissue environment to support human GCs from various blood sources.
  • - The new immune organoid systems maintain critical B cell functions longer and offer unique immune programming features; however, they show less effectiveness with lymphoma-derived B cells, indicating a rapid method for studying immune responses and B cell-related disorders.
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Housing is a critical social determinant of children's health. While individual pathways between housing and health are well evidenced, there has been less attention on the co-occurrence of housing disadvantages. We aim to identify typologies of children's housing disadvantage and describe the health inequities they generate.

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  • The systematic review investigated the impact of maxillofacial reconstruction with dental implants on quality of life (QoL), comparing it to no dental rehabilitation and other options like removable dentures.
  • After analyzing 2735 studies, only three were included, suggesting that maxillofacial reconstruction improved QoL, but they had a high risk of bias due to confounding factors.
  • The review found that existing QoL measurement tools were not sufficiently relevant for maxillofacial patients, emphasizing the need for tailored instruments to better assess their quality of life.
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The objective of this study was to examine the interactions between anionic surfactants, specifically 1-alkylsulfonates (KXS) and 1-alkylsulfates (SXS) ions, with human serum albumin (HSA). A combination of experimental techniques, including isothermal titration calorimetry (ITC), steady-state fluorescence spectroscopy (SF), and molecular dynamics-based approaches was employed to gain a comprehensive understanding of these processes. It has been demonstrated that the subtle variations in the charge distribution on the anionic surfactant headgroups have a significant impact on the number of binding sites, the stoichiometry of the resulting complexes, and the strength of the interactions between the surfactants and the protein.

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Objective: To assess the clinical and molecular profile of patients with Duchenne Muscular Dystrophy (DMD) presenting to a tertiary center in Eastern Region of Uttar Pradesh, India.

Methods: In this retrospective study, case records of all patients diagnosed as DMD were analyzed to ascertain the clinical phenotype and molecular profile. Multiplex polymerase chain reaction (mPCR) technique, Multiplex Ligation Dependent Probe Amplification (MLPA) and Next Generation Sequencing (NGS) were used for establishing the molecular diagnosis.

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Organic polymers have found diverse applications in the industrial and scientific world. One such application is using chitosan-based electrochemical sensors, which have gained rapid popularity due to their unique properties. To further enhance the material's porosity and adsorption capacity, the incorporation of a surfactant into the film has been explored.

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  • Hydrogels are crucial in fields like tissue engineering and drug delivery, but current options lack customizability and programmability, limiting their effectiveness for 3D tissue culture.
  • The study introduces innovative DNA-based hydrogel systems that address these limitations through a simple one-step synthesis method and the ability to fine-tune mechanical properties and network structure.
  • The newfound DNA hydrogels were shown to positively influence retinal pigment epithelial cells, enhancing aspects like shape, protein expression, and cell growth, suggesting potential for advanced applications in cellular programming and tissue engineering.
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Perfusable hydrogels have garnered substantial attention in recent years for the fabrication of microphysiological systems. However, current methodologies to fabricate microchannels in hydrogel platforms involve sophisticated equipment and techniques, which hinder progress of the field. In this protocol, we present a cost-effective, simple, versatile and ultrafast method to create perfusable microchannels of complex shapes in photopolymerizable hydrogels.

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  • - The study investigates how trace elements (like zinc and magnesium) relate to inflammation markers (TNF-α and IL-6) in children with short stature, aiming to identify potential causes for this condition.
  • - Researchers analyzed 220 samples, revealing lower levels of zinc and magnesium and higher copper levels in short-statured individuals, with a notable correlation between these trace elements and inflammation markers.
  • - The findings suggest that monitoring the Cu/Zn ratio and inflammation markers could help identify children at risk for short stature, and zinc supplementation might improve growth by reducing inflammation.
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Introduction: Australia's limited social housing has created geographically concentrated locales of poverty with high smoking rates. The impact of social housing on smoking initiation among adolescent residents is unknown, despite adolescence being a critical period for smoking prevention. We examine the relationship between social housing residency and smoking initiation amongst adolescents to quantify the likelihood of smoking uptake amongst social housing residents compared to a similar cohort in other tenures, accounting for socio-economic factors and household exposure to smoking.

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Secondary lymphoid organs (SLOs), including tonsils (TS), lymph nodes (LN), and Peyer's Patches, exhibit complementary immune functions. However, little is known about the spatial organization of immune cells and extracellular matrix (ECM) in the SLOs. Traditional imaging is limited to a few markers, confining our understanding of the differences between the SLOs.

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Background And Rationale: Dental care systems have the potential to influence population oral health and patterns of socioeconomic inequalities. Therefore, understanding the impact of the ways in which countries fund, provide, and organize their dental care services is key in the analysis of determinants of oral health. In this commentary we offer a synopsis of recent typologies of healthcare systems, based on a rapid review, and highlight that none of them fit dental care services given the separation of dental care from general healthcare provision in many countries.

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With the advent of DNA nanotechnology, DNA-based biomaterials have emerged as a unique class of materials at the center of various biological advances. Owing to DNA's high modification capacity programmable Watson-Crick base-pairing, DNA structures of desired design with increased complexity have been developed. However, the limited scalability, along with poor mechanical properties, high synthesis costs, and poor stability, reduced the adaptability of DNA-based materials to complex biological applications.

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Objective: High-risk human papillomaviruses (HPV) are an established cause of oropharyngeal cancer. Their relationship with oral cancer remains unclear with detection ranging from 0% to 100%. HPV DNA detection or evidence of exposure alone is insufficient to conclude causality.

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