Role of dental pulp in age estimation: A quantitative and morphometric study.

Context: This study was designed to characterize the role of the dental pulp (DP) in age estimation.

Aim: The analysis of age-related quantifiable changes in DP components such as odontoblasts, collagen fibers, and blood vessels.

Subjects And Methods: One hundred and twenty extracted teeth from six age groups (20-30 years, 31-40 years, 41-50 years, 51-60 years, 61-70 years, and 71-80 years) were subjected to decalcification and routine histopathological processing followed by Hematoxylin and Eosin and Picrosirius Red staining.

Modified Cajal's trichrome stain as a diagnostic aid in the study of epithelial pathology.

Background: Diagnosis of initial epithelial pathology maybe difficult in Squamous Cell Carcinoma (SCC), Carcinoma In Situ and other atypical epithelial malignancies, under routine Haematoxylin and Eosin (H and E) stain. The detection of minor basement membrane alterations in doubtful cases is both time consuming and confusing.

Aims: To evaluate efficacy of Modified Cajal's Trichrome Stain (CTS) in relation to Haematoxylin and Eosin for study of epithelial dysplasia, carcinoma in situ, micro invasive SCC, frank SCC, and SCC in lymph nodes.