Publications by authors named "Anjana Kaveri Badekila"
Therapeutic strategy for efficiently targeting cancer cells needs an in-depth understanding of the cellular and molecular interplay in the tumor microenvironment (TME). TME comprises heterogeneous cells clustered together to translate tumor initiation, migration, and proliferation. The TME mainly comprises proliferating tumor cells, stromal cells, blood vessels, lymphatic vessels, cancer-associated fibroblasts (CAFs), extracellular matrix (ECM), and cancer stem cells (CSC).
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- Natural polymeric hydrogels, particularly alginate/carboxymethylcellulose (Alg/CMC), are effective for creating 3D cell cultures, specifically for Huh-7 hepatocellular carcinoma cells using a freeze-thaw ionic crosslinking technique.
- The characterization of these hydrogels shows they have ideal properties like uniformity, appropriate pore size, viscoelasticity, and optimal degradation rates for supporting 3D culture.
- The study reveals that 3D spheroids reach a size of 250-300 μm by day 7, with gene expression analysis indicating significant upregulation of key proteins associated with cell interactions and maintaining epithelial characteristics during spheroid formation.
Background: Assessing an optimal reference gene as an internal control for target gene normalization is important during quantitative real time polymerase chain reaction (RT-qPCR) of three dimensional (3D) cell culture. Especially, gene profiling of cancer cells under a complex 3D microenvironment in a polymer scaffold provides a deeper understanding of tumor functioning in vivo.
Methods And Results: Expression of six housekeeping genes (HKG's): Glyceraldehyde-3-phosphodehydrogenase (GAPDH), β-actin (ACTB), beta-2-microglobulin (B2M), 18S ribosomal RNA (18S rRNA), peptidyl-propyl-isomerase A (PPIA), and ribosomal protein L13 (RPL-13) during two dimensional (2D) culture, and alginate-carboxymethylcellulose scaffold based 3D culture conditioned up to 21 days was analysed for hepatocellular carcinoma (Huh-7) cells.