Label-free imaging of cell attachment with photonic crystal enhanced microscopy.

We introduce photonic crystal enhanced microscopy (PCEM) as a label-free biosensor imaging technique capable of measuring cell surface attachment and attachment modulation. The approach uses a photonic crystal optical resonator surface incorporated into conventional microplate wells and a microscope-based detection instrument that measures shifts in the resonant coupling conditions caused by localized changes in dielectric permittivity at the cell-sensor interface. Four model systems are demonstrated for studying cancer cells, primary cardiac muscle cells, and stem cells.

Characterization of military fog oil by comprehensive two-dimensional gas chromatography.

The most commonly used military fog oil is characterized by comprehensive two-dimensional gas chromatography (GC x GC) coupled to either Flame Ionization Detection (FID) or Time-of-Flight Mass Spectrometric Detection (TOFMS) to advance the knowledge regarding the complete chemical makeup of this complex matrix. Two different GC x GC column sets were investigated, one employing a non-polar column combined with a shape selective column and the other an inverse column set (medium-polar/non-polar). The inverse set maximizes the use of the two-dimensional separation space and segregates aliphatic from aromatic fractions.