Direct chromogenic substrate immuno-capture activity assay for testing of factor VII-activating protease.

Background: The Marburg I (MRI) single nucleotide polymorphism (SNP) of the factor VII-activating protease (FSAP) gene has been associated with thrombophilia and atherosclerotic disease. PCR is used to detect the SNP. Also, the specific FSAP activity to cleave single-chain urokinase-type plasminogen activator (scu-PA) serves as a surrogate for PCR testing.

Coagulation assays based on the Luminescent Oxygen Channeling Immunoassay technology 1).

Background: The Luminescent Oxygen Channeling Immunoassay (LOCI) technology is a well-established homogeneous assay format that allows for fast, accurate, and highly sensitive quantitation of analytes. We set out to develop and prove a novel concept to establish a LOCI format that should principally allow for the determination of the activity of coagulation factors and anticoagulants of clinical relevance.

Methods: The concept is based on the linkage of LOCI nano-beads by a peptide that can be cleaved by a coagulation factor.