Publications by authors named "Anja Gulliksen"
Article Synopsis
- The paper discusses a hands-free diagnostic platform designed to detect HPV E6/E7 mRNA in clinical samples, featuring automated processes for sample preparation and detection.
- It includes two modular components: one for preparing samples and another for amplification and detection, with plans for future integration into a single unit.
- Testing results showed a detection rate of 50%-85.7% for HPV in cervical cytology samples, with successful amplification of specific HPV types in some cases.
Cancer affects more people than any other disease. About one-third of the world's population is likely to get this diagnosis during their lifetime. Currently, the diagnostic methods for cancer detection are based on visual inspection.
View Article and Find Full Text PDFA general multipurpose microchip technology platform for point-of-care diagnostics has been developed. Real-time nucleic acid sequence-based amplification (NASBA) for detection of artificial human papilloma virus (HPV) 16 sequences and SiHa cell line samples was successfully performed in cyclic olefin copolymer (COC) microchips, incorporating supply channels and parallel reaction channels. Samples were distributed into 10 parallel reaction channels, and signals were simultaneously detected in 80 nl volumes.
View Article and Find Full Text PDFReal-time nucleic acid sequence-based amplification (NASBA) is an isothermal method specifically designed for amplification of RNA. Fluorescent molecular beacon probes enable real-time monitoring of the amplification process. Successful identification, utilizing the real-time NASBA technology, was performed on a microchip with oligonucleotides at a concentration of 1.
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