Formulation of Ready-to-Use Broccoli Extracts Rich in Polyphenols and Glucosinolates Using Natural Deep Eutectic Solvents.

Broccoli is rich in biologically active compounds, especially polyphenols and glucosinolates, known for their health benefits. Traditional extraction methods have limitations, leading to a shift towards using natural deep eutectic solvents (NADESs) to create high-quality extracts with enhanced biological activity. This study focuses on preparing broccoli extracts in NADES, enriched with polyphenols and glucosinolates, without additional purification steps.

Harnessing the potential of deep eutectic solvents in biocatalysis: design strategies using CO to formate reduction as a case study.

Introduction: Deep eutectic solvents (DESs) have emerged as green solvents with versatile applications, demonstrating significant potential in biocatalysis. They often increase the solubility of poorly water-soluble substrates, serve as smart co-substrates, modulate enzyme stereoselectivity, and potentially improve enzyme activity and stability. Despite these advantages, screening for an optimal DES and determining the appropriate water content for a given biocatalytic reaction remains a complex and time-consuming process, posing a significant challenge.

Sustainable isolation of ginger (Zingiber officinale) herbal dust bioactive compounds with favorable toxicological profile employing natural deep eutectic solvents (NADES).

The usage of ginger (Zingiber officinale) has increased in recent years due to its positive effect on human health affiliated with its richness in gingerols and shogaols. This study optimized the Ultrasound-assisted extraction (UAE) for better phenolic compounds isolation from ginger herbal dust (GHD), a filter tea industry by-product. The extraction was performed using raw and defatted GHD-previously processed by Supercritical fluid extraction - CO.

Enhancing protein stability under stress: osmolyte-based deep eutectic solvents as a biocompatible and robust stabilizing medium for lysozyme under heat and cold shock.

In biomedical and biotechnological domains, liquid protein formulations are vital tools, offering versatility across various fields. However, maintaining protein stability in a liquid form presents challenges due to environmental factors, driving research to refine formulations for broader applications. In our recent study, we investigated the relationship between deep eutectic solvents (DESs) and the natural presence of osmolytes in specific combinations, showcasing the effectiveness of a bioinspired osmolyte-based DES in stabilizing a model protein.