Maturation of barley cysteine endopeptidase expressed in Trichoderma reesei is distorted by incomplete processing.

Maturation of barley cysteine endopeptidase B (EPB) in Trichoderma reesei was studied with metabolic in hibitors, Western blotting, and immuno microscopy. The inactive 42-kDa recombinant EPB proprotein, first detected in apical cells, was sequentially processed in a time-dependent manner to a secreted polypeptide of 38.5 kDa, and thereafter, to polypeptides of 37.

Glutamine synthetase isozymes in germinating barley seeds.

Glutamine synthetase (GS; EC 6.3.1.