An optimized micro-X-ray fluorescence confocal imaging (μXRF-CI) analytical method has been developed to determine the 2D distribution of elemental composition in small (1-3 mm) biological objects at a 10-20 μm spatial resolution. Plants take up chemical elements from soil, and the vascular system transports them toward shoots. In order to obtain biochemical information related to this biological process, 2D distributions of chemical elements in roots and in hypocotyls of cucumber plants were analyzed by synchrotron radiation based on micro-X-ray fluorescence computer tomography and μXRF-CI techniques.
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