Live Plague Vaccine Development: Past, Present, and Future.

During the last 100 years, vaccine development has evolved from an empirical approach to one of the more rational vaccine designs where the careful selection of antigens and adjuvants is key to the desired efficacy for challenging pathogens and/or challenging populations. To improve immunogenicity while maintaining a favorable reactogenicity and safety profile, modern vaccine design must consider factors beyond the choice of target antigen alone. With new vaccine technologies currently emerging, it will be possible to custom-design vaccines for optimal efficacy in groups of people with different responses to vaccination.

Genetically Engineered Bacterial Ghosts as Vaccine Candidates Against Infection.

Bacterial ghosts (BGs), non-living empty envelopes of bacteria, are produced either through genetic engineering or chemical treatment of bacteria, retaining the shape of their parent cells. BGs are considered vaccine candidates, promising delivery systems, and vaccine adjuvants. The practical use of BGs in vaccine development for humans is limited because of concerns about the preservation of viable bacteria in BGs.

Evaluation of 16S rRNA genes sequences and genome-based analysis for identification of non-pathogenic .

16S rRNA genes sequencing has been used for routine species identification and phylogenetic studies of bacteria. However, the high sequence similarity between some species and heterogeneity within copies at the intragenomic level could be a limiting factor of discriminatory ability. In this study, we aimed to compare 16S rRNA genes sequences and genome-based analysis (core SNPs and ANI) for identification of non-pathogenic .

NR1D1 Inhibition Enhances Autophagy and Mitophagy in Alzheimer's Disease Models.

Alzheimer's disease (AD) is marked by extracellular beta-amyloid (Aβ) plaques and intracellular Tau tangles, leading to progressive cognitive decline and neuronal dysfunction. Impaired autophagy, a process by which a cell breaks down and destroys damaged or abnormal proteins and other substances, contributes to AD progression. This study investigated Nuclear Receptor Subfamily 1 Group D Member 1 (NR1D1) as a potential therapeutic target for modulating autophagy.

Loss-of-function mutations of the TIE1 receptor tyrosine kinase cause late-onset primary lymphedema.

Primary lymphedema (PL), characterized by tissue swelling, fat accumulation, and fibrosis, results from defects in lymphatic vessels or valves caused by mutations in genes involved in development, maturation, and function of the lymphatic vascular system. Pathogenic variants in various genes have been identified in about 30% of PL cases. By screening of a cohort of 755 individuals with PL, we identified two TIE1 (tyrosine kinase with immunoglobulin- and epidermal growth factor-like domains 1) missense variants and one truncating variant, all predicted to be pathogenic by bioinformatic algorithms.

Room Temperature Ultrafast Oxidative Desulfurization with Sodium Hypochlorite in the Presence of Silica-Supported Catalysts.

A series of silica-supported catalysts containing molybdenum, tungsten, and vanadium oxides as the active phase was investigated in the process of oxidative desulfurization with sodium hypochlorite. It was shown for the first time that catalysts containing vanadium oxide as the active phase are more stable under oxidation conditions with sodium hypochlorite and retain their effectiveness at increased dosages of the oxidant and at high initial sulfur contents. The catalysts were characterized in detail by a complex of methods: Fourier transform infrared, X-ray spectral fluorescence, transmission electron microscopy, scanning electron microscopy, and low-temperature nitrogen adsorption/desorption.

Charge-compensated -carborane derivatives in the synthesis of iron(II) bis(dicarbollide) complexes.

A series of stable iron(II) bis(dicarbollide) derivatives [8,8'-(RNHC(Et)HN)-3,3'-Fe(1,2-CBH)] (R = Pr, R = Ph, (CH)OH, (CH)OH, (CH)NMe) was prepared starting from FeCl or [FeCl(dppe)] and the corresponding -carboranyl amidines [10-RNHC(Et)HN-7,8-CBH]. In a similar way, the reactions of the oxonium derivatives of -carborane with FeCl in tetrahydrofuran in the presence of -BuOK lead to the corresponding stable oxonium derivatives iron(II) bis(dicarbollide) [8,8'-(RR'O)-3,3'-Fe(1,2-CBH)] (RR' = (CH), (CH)O(CH), (CH); R = R' = Et), which can be alternatively prepared by the reaction of the parent iron(II) bis(dicarbollide) with tetrahydrofuran or 1,4-dioxane in the presence of MeSO. The cyclic voltammetry studies of the synthesized iron(II) bis(dicarbollide) derivatives revealed that the introduction of amidinium and oxonium substituents leads to a significant increase in the Fe/Fe redox potential relative to the parent iron(II) bis(dicarbollide).

Electron density-based protocol to recover the interacting quantum atoms components of intermolecular binding energy.

A new approach for obtaining interacting quantum atoms-defined components of binding energy of intermolecular interactions, which bypasses the use of standard six-dimensional integrals and two-particle reduced density matrix (2-RDM) reconstruction, is proposed. To examine this approach, three datasets calculated within the density functional theory framework using the def2-TZVP basis have been explored. The first two, containing 53 weakly bound bimolecular associates and 13 molecular clusters taken from the crystal, were used in protocol refinement, and the third one containing other 20 bimolecular and three cluster systems served as a validation reference.

[Forensic expert evaluation of adverse outcomes of health care delivery in criminal proceedings].

Is to analyze the historical development and current state of the Institute of Commission Forensic Medical Examination of adverse outcomes of health care delivery in the domestic criminal proceedings. The article describes the main historical periods of development, summarizes the main problems and identifies the place of commission forensic medical examination for medical cases in current system of forensic medical activity.

[Experimental study of the effectiveness of terbinafine in the treatment of fungal keratitis].

Unlabelled: Methods of treatment of fungal keratitis in the Russian Federation currently consist of non-standardized off-label methods due to the lack of licensed commercial antifungal solutions designed for use in ophthalmology. The article presents the results of successful treatment of fungal keratitis in experimental animals with a terbinafine solution, with effectiveness comparable to voriconazole lyophilizate diluted to 1%.

Purpose: The study analyzed the effectiveness of terbinafine solution in the experimental treatment of fungal keratitis.

Ladder Polyphenylsilsesquioxanes and Their Niobium-Siloxane Composite as Coating Materials: Spectroscopy and Atomic Oxygen Resistance Study.

In order to expand the range of materials that can be used in outer space and in development of small spacecraft, ladder polyphenylsilsesquioxanes with different molar weights and the Nb-siloxane composites based on them were studied. The properties of the polymer films were studied, including tests in an oxygen plasma flow. Both initial and filled ladder polymers feature extremely low erosion coefficients in the region of 10 cm/atom O at a high fluence of atomic oxygen of 1.

Pervaporation and Gas Separation Properties of High-Molecular Ladder-like Polyphenylsilsesquioxanes.

This paper presents the results of studies on the pervaporation properties (for benzene/hexane mixtures) and gas permeability (for He, H, N, O, CO, CH, CH, and CH) of ladder-like polyphenylsesquioxanes (L-PPSQ) with improved physical and chemical properties. These polymers were obtained by condensation of -tetraphenylcyclotetrasiloxanetetraol in ammonia medium. The structure of L-PPSQ was fully confirmed by a combination of physicochemical analysis methods: H, Si NMR, IR spectroscopy, HPLC, powder XRD, and viscometry in solution.

Synthesis of Tungsten Carbides in a Copper Matrix by Spark Plasma Sintering: Microstructure Formation Mechanisms and Properties of the Consolidated Materials.

In this study, the synthesis of tungsten carbides in a copper matrix by spark plasma sintering (SPS) is conducted and the microstructure formation mechanisms of the composite materials are investigated. The reaction mixtures were prepared by the high-energy mechanical milling (MM) of W, C and Cu powders. The influence of the MM time and SPS temperature on the tungsten carbide synthesis in an inert copper matrix was analyzed.

The angiopoietin receptor Tie2 is atheroprotective in arterial endothelium.

Leukocytes and resident cells in the arterial wall contribute to atherosclerosis, especially at sites of disturbed blood flow. Expression of endothelial Tie1 receptor tyrosine kinase is enhanced at these sites, and attenuation of its expression reduces atherosclerotic burden and decreases inflammation. However, Tie2 tyrosine kinase function in atherosclerosis is unknown.

Tetrachromophoric Derivatives of Dibenzoylmethanatoboron Difluoride Based on Stereoregular Cyclotetrasiloxanes: Synthesis and Properties.

A series of new tetrachromophoric systems based on stereoregular tetracyclosiloxanes and dibenzoylmethanatoboron difluoride derivatives have been synthesized and characterized by a complex of physicochemical methods. The photophysical properties of the synthesized compounds are studied by electronic absorption, steady-state, and time-resolved fluorescence spectroscopy. In the synthesized compounds, four dibenzoylmethanatoboron difluoride (DBMBF)-based fluorophores are in an all-cis arrangement with respect to a cyclotetrasiloxane scaffold.

Synthesis of Siloxane Derivatives of Phenylboronic Acids of Different Structures.

The synthesis of a storage-stable organosilicon modifier with a dioxaborolane-protecting group is described. Its high reactivity and selective anti-Markovnikov addition in hydrosilylation reactions to afford siloxanes of various structures are shown. The possibility of deprotection of both the initial modifier and its siloxane derivatives under mild conditions using water in yields up to 96% is demonstrated.

Impact of regiochemistry in energetic materials science: a case of (nitratomethyl-1,2,3-triazolyl)furazans.

The preparation of multipurpose high-energy materials for space technologies remains a challenging task and such materials usually require special precautions and fine tunability of their functional properties. To unveil new opportunities to high-performance energetic materials, novel potential melt-castable explosives and energetic plasticizers incorporating a (1,2,3-triazolyl)furazan scaffold enriched with nitro and nitratomethyl explosophoric functionalities were synthesized. The successful implementation of the regiodivergent approach enabled the preparation of regioisomeric (nitratomethyltriazolyl)furazans that possessed significantly different physicochemical properties classifying the target materials as melt-castable substances or energetic plasticizers.

Optical Characterization of Thin Films by Surface Plasmon Resonance Spectroscopy Using an Acousto-Optic Tunable Filter.

The paper presents the application of the acousto-optic tunable filter (AOTF) in surface plasmon resonance (SPR) spectroscopy to measure the optical thickness of thin dielectric coatings. The technique presented uses combined angular and spectral interrogation modes to obtain the reflection coefficient under the condition of SPR. Surface electromagnetic waves were excited in the Kretschmann geometry, with the AOTF serving as a monochromator and polarizer of light from a white broadband radiation source.

Yersinia pestis Surface Antigens in Reception of Specific Bacteriophages.

The significance of Yersinia pestis surface antigens in adhesiveness to specific bacteriophages has been studied with the use of two methodological approaches. It was shown that Ail protein immobilized on the surface of polystyrene microspheres (but not in the solution), can bind both the Pokrovskaya phage and pseudotuberculous diagnostic phage. YapF autotransporter interacted with both phages in a water-soluble form, but YapF bound to polystyrene microspheres interacted only with the Pokrovskaya phage.

Measuring of water transport selectively along the plant root plasmodesmata using gradient nuclear magnetic resonance with paramagnetic doping.

In this study, we measured translational water diffusion selectively along symplast pathway through plasmodesmata in maize roots, and the effective plasmodesmata permeability coefficient (P) was determined using a nuclear magnetic resonance (NMR) spin echo method. Measuring of water transport selectively along the plant root plasmodesmata was achieved with paramagnetic complexes (PCs) of high relaxation efficiency. PCs penetrate into the intercellular space of root tissue, but not into cells, and accelerate the magnetic relaxation processes of intercellular water, thereby excluding the contribution of intercellular water to the registered NMR diffusion echo attenuation.

Sulfur containing mixed wastes in anaerobic processing by new immobilized synthetic consortia.

Methanogenic biotransformation of unusual substrates (sulfur (S)-containing wastes: non-purified vacuum gas oil, straight-run gasoline fraction (Naphtha), gas condensate, and straight-run diesel fraction) coming from oil industry after their oxidative desulfurization was investigated. Nitrogen-containing wastes (hydrolysates of chicken manure and Chlorella vulgaris biomass) were added as co-substrates to mixture with oil industry wastes. The 100 % conversion of S-organic compounds to inorganic sulfide accumulated in the reaction liquid medium was achieved with simultaneous production of biogas containing high methane percent (greater than 70 %).

The Use of the Thiol-Ene Addition Click Reaction in the Chemistry of Organosilicon Compounds: An Alternative or a Supplement to the Classical Hydrosilylation?

This review presents the main achievements in the use of the thiol-ene reaction in the chemistry of silicones. Works are considered, starting from monomers and ending with materials.The main advantages and disadvantages of this reaction are demonstrated using various examples.

Lymphangiogenesis requires Ang2/Tie/PI3K signaling for VEGFR3 cell-surface expression.

Vascular endothelial growth factor C (VEGF-C) induces lymphangiogenesis via VEGF receptor 3 (VEGFR3), which is encoded by the most frequently mutated gene in human primary lymphedema. Angiopoietins (Angs) and their Tie receptors regulate lymphatic vessel development, and mutations of the ANGPT2 gene were recently found in human primary lymphedema. However, the mechanistic basis of Ang2 activity in lymphangiogenesis is not fully understood.

Strengthen climate adaptation research globally.

More international incentives and coordination are needed.