A novel gellan-based nanoemulgel delivery system for co-encapsulation and in vitro digestion of hydrophilic/hydrophobic nutraceuticals.

Preventive healthcare strategies are gaining attention over traditional approach of treating and managing diseases. The use of food hydrocolloids has garnered interest in developing innovative food formulations promoting healthy eating habits. Among emerging carrier systems, nanoemulgel holds significant potential with its ability to deliver hydrophilic and lipophilic nutraceuticals through a combination of nanoemulsion and hydrogel technology.

Microbial fermentation-based synthesis of nano-curcumin suggesting the role of pullulan in nano-formulation.

Curcumin is a multitargeting nutraceutical with numerous health benefits, however, its efficacy is limited due to poor aqueous solubility and reduced bioavailability. While nano-formulation has emerged as an alternative to encounter such issues, it often involves use of toxic solvents. Microbial synthesis may be an innovative solution to address this lacuna.

Thermo-reversible self-assembled novel gellan gum hydrogels containing amino acid biogelators with antibacterial activity.

In recent years, hydrogels derived from natural polymers have gained considerable attention. However, lack of mechanical strength and poor stability has become major lacuna of such systems. Scientists have attempted to resolve this problem by introducing chemical cross-linkers or synthetic modifications of natural polymers.

Self-assembled pH-stable gellan/κ-carrageenan bigel: Rheological studies and viscosity prediction by neural network.

The current study focused on analysing and predicting the effect of physicochemical parameters on the rheological properties of the novel polysaccharide-based bigel. This is the first study to report a bigel fabricated entirely from polysaccharides and develop a neural network to predict the modulation in its rheology. This bi-phasic gel had gellan and κ-carrageenan as the constitutive elements in the aqueous and the organic phase, respectively.

Enhanced encapsulation efficiency and controlled release of co-encapsulated Bacillus coagulans spores and vitamin B9 in gellan/κ-carrageenan/chitosan tri-composite hydrogel.

The current study, for the first time, attempts to co-encapsulate Bacillus coagulans spores as probiotics and vitamin B9 in the polysaccharide-based matrix for their targeted delivery. Instead of vegetative cells, probiotic spores were chosen owing to their higher stability. The matrix, tri-composite hydrogel, was synthesized from gellan, κ-carrageenan, and chitosan through self-assembly devoid of chemical cross-linkers.

Microbial Polysaccharide-Based Nanoformulations for Nutraceutical Delivery.

In recent times, nutrition and diet have become prominent health paradigms due to sedentary lifestyle disorders. Preventive health care strategies are becoming increasingly popular instead of treating and managing diseases. A nutraceutical is an innovative concept that offers additional health benefits beyond its fundamental nutritional value.

Isolation, purification, and characterization of a novel exopolysaccharide isolated from marine bacteria Brevibacillus borstelensis M42.

Marine microbes produce polysaccharides with unique physicochemical and functional properties that help them survive in harsh marine environments. However, only a handful of marine exopolysaccharides (EPSs) have been reported to date. The present study explored the seashore of Visakhapatnam, India, to report a novel exopolysaccharide designated as Br42 produced by Brevibacillus borstelensis M42.

Green Synthesis of pH-Responsive, Self-Assembled, Novel Polysaccharide Composite Hydrogel and Its Application in Selective Capture of Cationic/Anionic Dyes.

Dyes are one of the most hazardous chemicals causing significant environmental pollution and affecting water quality. Majority of the existing methods for dye removal and degradation involve synthetic membranes and use of hazardous chemicals, further resulting in secondary pollution. The present study reports polysaccharide based novel composite hydrogel as biodegradable matrix for pH-responsive selective adsorption of cationic/anionic dyes.

Recent advances in composite hydrogels prepared solely from polysaccharides.

The proliferating demand for sustainable, biodegradable, and biologically safe materials has triggered the development of polysaccharide-based hydrogels. The translation of research on single polysaccharide-based hydrogels into their desired clinical or industrial application is minimal. This is attributable to their lack of mechanical strength, inadequate stability, and constrained the possibility of their modulation to obtain the desired property.

Synthesis and rheological characterization of a novel shear thinning levan gellan hydrogel.

Shear thinning hydrogels are majorly employed in biomedical and cosmetic industry. However, the application of such chemically modified hydrogels is limited due to their high gelation time and chemical toxicity. The present study attempts to address these issues by synthesizing a novel quick setting, non-toxic hydrogel composed of biodegradable polysaccharides, levan and gellan.

pH mediated rheological modulation of chitosan hydrogels.

Chitosan (CS) hydrogels are used widely for multifarious applications in diverse fields due to the property of pH responsiveness. This study aims to explain the sensitivity of CS hydrogels to alteration in pH due to change in the concentration of acetic acid, which is a solvent for CS. Studies on changes in rheological properties in this regard are still scarce.

Exploration of polysaccharide based nanoemulsions for stabilization and entrapment of curcumin.

This study was focussed on developing polysaccharide based nanoemulsion (P-NE) encapsulating curcumin. Two different carrier oil, olive and castor oil, and various polysaccharides like, levan, fucoidan, alginate, guar gum and κ-carrageenan were evaluated for their entrapment efficiency for curcumin. The prepared nanoemulsions with fucoidan and κ-carrageenan were found to have relatively better properties than nanoemulsions prepared with Tween 20.

Understanding the Effect of Impeller Configurations on Pullulan Production by RBF 4A3.

Mass transfer is one of the most important factors involved in viscous fermentation processes, like production of pullulan. Impellers play a crucial role in maintaining homogeneity and better mass transfer conditions during the fermentation process. The present study attempted to evaluate the efficiency of impellers with diverse configurations during pullulan fermentation.

Synthesis of a novel gellan-pullulan nanogel and its application in adsorption of cationic dye from aqueous medium.

The current study focused on synthesizing a novel gellan-pullulan composite nanogel via chemical crosslinking with DPPH. The physical and rheological properties of the synthesised nanogel was assessed by determining its particle size, zeta potential and effect of temperature and stress on viscosity of nanogels. Furthermore, the novel nanogel was assessed for its ability to ameliorate water resources by efficiently adsorbing cationic dyes.

Synthesis and rheological studies of a novel composite hydrogel of xanthan, gellan and pullulan.

Hydrogels are three dimensional polymeric structures and are of great importance to industrial sector due to their signature physiochemical and mechanical properties. Use of natural polysaccharides as the components of hydrogel composite is of great interest due to their non-toxic nature, biodegradability, biocompatibility and viscoelastic properties. The present study reports a novel hydrogel composed of xanthan, gellan and pullulan.

Purification, characterization and functional properties of exopolysaccharide from a novel halophilic Natronotalea sambharensis sp. nov.

An exopolysaccharide producing strain AK103 was isolated from Sambharlake, Rajasthan, India. Based on polyphasic taxonomy, the strain AK103 was found to be a novel species for which the name Natronotalea sambharensis sp. nov.

A review on the biosynthesis of metal and metal salt nanoparticles by microbes.

Metal nanoparticles have received great attention from researchers across the world because of a plethora of applications in agriculture and the biomedical field as antioxidants and antimicrobial compounds. Over the past few years, green nanotechnology has emerged as a significant approach for the synthesis and fabrication of metal nanoparticles. This green route employs various reducing and stabilizing agents from biological resources for the synthesis of nanoparticles.

Structural characterization and antioxidant potential of a novel anionic exopolysaccharide produced by marine Microbacterium aurantiacum FSW-25.

An exopolysaccharide (EPS) producing strain FSW-25 was isolated from the Rasthakaadu beach Kanyakumari, Tamil Nadu India. Based on polyphasic taxonomy, the strain FSW-25 was assigned to the genus Microbacterium and found to be the closest relative of the species aurantiacum. Large quantity of EPS (7.

Production, characterization and bio-emulsifying activity of a novel thermostable exopolysaccharide produced by a marine strain of Rhodobacter johrii CDR-SL 7Cii.

An exopolysaccharide (EPS) producing bacterial strain was isolated from the surface of marine macroalgae (Padina sp.). Based on polyphasic taxonomy, the strain CDR-SL 7Cii was assigned to the genus Rhodobacter and found to be the closest relative of the species Rhodobacter johrii.

Synthesis and rheological characterization of a novel thermostable quick setting composite hydrogel of gellan and pullulan.

Hydrogels have found applications in diverse industrial sectors and majority of them are chemically modified. However, recently demand for stable biocompatible non-toxic hydrogels has increased significantly. The current study is focused on synthesizing a novel composite hydrogel using gellan and pullulan, two commercially available biologically safe polysaccharides, without any chemical modifications.

Microbial glycolipoprotein-capped silver nanoparticles as emerging antibacterial agents against cholera.

Background: With the increased number of cholera outbreaks and emergence of multidrug resistance in Vibrio cholerae strains it has become necessary for the scientific community to devise and develop novel therapeutic approaches against cholera. Recent studies have indicated plausibility of therapeutic application of metal nano-materials. Among these, silver nanoparticles (AgNPs) have emerged as a potential antimicrobial agent to combat infectious diseases.

Extracellular polysaccharide production by a novel osmotolerant marine strain of Alteromonas macleodii and its application towards biomineralization of silver.

The present study demonstrates exopolysaccharide production by an osmotolerant marine isolate and also describes further application of the purified polysaccharide for production of colloidal suspension of silver nanoparticles with narrow size distribution. Phylogenetic analysis based on 16S r RNA gene sequencing revealed close affinity of the isolate to Alteromonas macleodii. Unlike earlier reports, where glucose was used as the carbon source, lactose was found to be the most suitable substrate for polysaccharide production.

Facile and rapid thermo-regulated biomineralization of gold by pullulan and study of its thermodynamic parameters.

A novel method for the production of gold nanoparticles (AuNPs) using pullulan as reducing and stabilizing agent has been developed. Quasi-spherical shaped AuNPs in the range of 50-100 nm were produced at three different temperature regimes 80°C, 90°C and 100°C as characterized using UV-vis spectrophotometer, TEM and DLS. Study of reaction kinetics and thermodynamic parameters indicated that the reaction between pullulan and chloroauric acid for AuNPs formation followed first order reaction kinetics and higher temperature was favorable for the synthesis of smaller sized AuNPs.

Cost effective production of pullulan from agri-industrial residues using response surface methodology.

Response surface methodology was used to develop an economically feasible process for the fermentative production of pullulan using agri-industrial residues, jaggery, de-oiled jatropha seed cake (DOJSC) and corn steep liquor (CSL), as sole media components. A second order polynomial model was obtained using central composite design to understand the effects of interactions among these substrates on pullulan biosynthesis. Results indicated that, lower concentrations of CSL and DOJSC and higher concentrations of jaggery favoured pullulan production.

Development of suitable solvent system for downstream processing of biopolymer pullulan using response surface methodology.

Downstream processing is an important aspect of all biotechnological processes and has significant implications on quality and yield of the final product. Several solvents were examined for their efficacy on pullulan precipitation from fermentation broth. Interactions among four selected solvents and their effect on pullulan yield were studied using response surface methodology.