Publications by authors named "Anil Kumar Nehra"
Article Synopsis
- A genetic tree revealed a major clade (BvG1) with a separate Taiwanese isolate identified as a new genotype (BvG2), showing some genetic variation between them (92.5-93.8% nucleotide identity).
- The study found high haplotype diversity among B. vogeli populations, particularly in India, with geographical structuring observed, where most haplotypes were country-specific, indicating limited genetic sharing between regions.
Article Synopsis
- The study focused on the genetic analysis of the fish species B. vogeli using cytochrome b gene sequences to understand its population structure.
- Phylogenetic analysis revealed that all B. vogeli sequences belong to a single group but are split into two subclades (Bv1 and Bv2), with several nucleotide variations leading to amino acid changes.
- The study found low genetic diversity within B. vogeli populations, confirmed a geographical distinction between Indian and Chinese populations, and suggested a stable population size based on neutrality tests.
Introduction: Equine theileriosis, an economically important disease that affects horses and other equids worldwide, is caused by a tick-borne intracellular apicomplexan protozoa . Genotyping of based on the 18S rRNA gene revealed the presence of two, three, four or five genotypes. In previous published reports, these genotypes have been labelled either alphabetically or numerically, and there is no uniformity in naming of these genotypes.
View Article and Find Full Text PDFThe present study was aimed to determine the phylogenetic relationship, haplotype network, and demographic dynamics of H. felis infecting the endangered Asiatic lions in Gir National Park, Gujarat, India, on the basis of partial 18S rRNA gene. The phylogenetic analysis based on the partial 18S rRNA gene sequences of H.
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- - The study focused on analyzing the genetic diversity of *Theileria annulata* using the cytochrome b gene, examining samples from various countries, including India, to assess population structure and buparvaquone resistance.
- - A maximum likelihood tree showed all analyzed *T. annulata* sequences grouped in one clade, but most haplotypes were unique to specific countries, indicating significant genetic differentiation with low gene flow between populations.
- - While all *T. annulata* isolates remained sensitive to buparvaquone, two new mutations in the cyt b gene associated with drug resistance were uncovered for the first time, highlighting potential concerns for treatment efficacy.
Background: There had been isolated reports of the presence of novel Theileria annulata genotypes based on the 18S rRNA gene sequence data from India, Pakistan and Saudi Arabia; but, these studies were restricted to limited field samples. Additionally, no comparative study has been conducted on all the isolates of this parasite from different countries whose sequences are available in the nucleotide databases. Therefore, we aimed to study the genetic diversity of T.
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- The study focused on analyzing the genetic diversity and relationships of Toxocara cati, a type of roundworm, using the ITS region of its DNA, identifying four distinct genotypes.
- Researchers found significant genetic differences between these genotypes and observed that China has the highest genetic variation among the studied locations.
- The findings suggest that T. cati populations have either remained stable or slightly increased in size over time, marking the first detailed genotyping effort for this species.
Article Synopsis
- - The study investigated the genetic diversity and haplotype network of *Theileria annulata* using 52 nearly complete 18S rRNA gene sequences from GenBank™, identifying 34 haplotypes with Haplotype 4 being the most widespread across countries like India, China, Turkey, and Iran.
- - India had the highest number of haplotypes (25) while Turkey, China, Iran, and Italy had fewer; the analysis found no geographical clustering of haplotypes, indicating high gene flow among populations.
- - The overall genetic analysis showed low nucleotide diversity but high haplotype diversity, suggesting recent population expansion and greater genetic variation within countries compared to between them.
The present study describes the genetic diversity in the Tams1 gene (733 bp) of Theileria annulata along with the sequence, phylogenetic and haplotype analyses of the Indian isolates. The phylogenetic analyses displayed distinct clustering of the Indian isolates into three groups suggesting the presence of three genotypes, hitherto designated as T. annulata genotypes 1-3 (G1-G3).
View Article and Find Full Text PDFThe present investigation was aimed to study the presence of Babesia caballi clades upon phylogenetic analysis of all available V4 hypervariable 18S rRNA gene sequences in GenBank in addition to the intra- and interclade genetic diversity in B. caballi and the distribution of parasite clades in different countries. Out of altogether 155 small-subunit ribosomal RNA gene sequences of B.
View Article and Find Full Text PDFHeat stress is an important environmental determinant which adversely affects the performance of poultry worldwide. The present communication reviews the impact of heat stress on production, reproduction and growth performance of poultry, and its alleviation using genetic strategies. The adverse effects of high environmental temperature on poultry include decrease in growth rate, body weight, egg production, egg weight, egg quality, meat quality, semen quality, fertility and hatchability, which cause vast financial losses to the poultry industry.
View Article and Find Full Text PDFA total of 57 tissue samples of domestic pigs (Sus scrofa) were collected from the meat outlets of five north Indian states and examined for sarcocystosis by histological and molecular methods. The genomic DNA extracted from five representative positive isolates was subjected to PCR amplification of the partial 18S rRNA gene followed by cloning and sequencing. Sequence analysis of the newly generated Indian isolates recorded 96.
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