Polymorphism in spa gene of Staphylococcus aureus from bovine subclinical mastitis.

Aim: The virulence-associated protein-A of Staphylococcus aureus, encoded by spa gene shows a variation in length in different strains. In this study, the spa gene variation in S. aureus strains was studied which were isolated from subclinical cases of bovine mastitis.

Typing of Staphylococcus aureus obtained from mastitic milk of cattle and buffalo on the basis of two virulence-associated genes (spa and clfA).

Aim: The present study was undertaken to type Staphylococcus aureus isolates from cattle and buffalo mastitic milk on the basis of spa (X-region) and clfA genes, both responsible for producing virulence factors.

Material And Methods: In the present investigation S. aureus isolates were isolated as per standard protocols.