Publications by authors named "Anil Kalia"
Article Synopsis
- Botulinum neurotoxin type A (BoNT/A) is being explored as a treatment for neuropathic pain, but its exact mechanism of action is still not well understood.* -
- Recent research focused on how BoNT/A interacts with voltage-gated sodium channels, particularly Nav1.7 and Nav1.3, using different experimental systems, including human-derived sensory neurons.* -
- Findings indicated that BoNT/A and its components had limited impact on sodium channel function and did not alter activity in sensory neurons from both healthy individuals and those with a genetic pain condition, suggesting other mechanisms may underlie its pain-relieving effects.*
Article Synopsis
- Human induced pluripotent stem cell (iPSC)-derived sensory neurons are important for modeling human diseases and for applications in drug discovery and regenerative medicine, especially in studying peripheral sensory neuropathies, which can lead to disorders like loss of pain or severe pain.* ! -
- The study compares two differentiation protocols: the newly developed "Anatomic" protocol, which produces a purer culture of sensory neurons, versus the "Chambers" protocol, which results in a mixed cell type population and predominantly tonic firing neurons.* ! -
- Results showed that patient-derived nociceptors from both protocols had higher firing frequencies compared to control neurons, with Anatomic demonstrating improved reproducibility and functionality in disease modeling.* !
Article Synopsis
- The study focuses on improving the differentiation of human induced pluripotent stem cell (iPSC)-derived peripheral sensory neurons for disease modeling and potential therapeutic applications.
- It compares two differentiation protocols: the newly developed "Anatomic" protocol, which yields purer neuronal cultures without toxic substances, and the traditional "Chambers" protocol, which results in mixed cell types.
- Results showed that the Anatomic protocol produced functionally active sensory neurons, including patient-derived nociceptors that had heightened firing frequencies, making them more viable for studying conditions related to pain.
The role of calcium release-activated calcium (CRAC) channels is well characterized and is of particular importance in T-cell function. CRAC channels are involved in the pathogenesis of several autoimmune diseases, making it an attractive therapeutic target for treating inflammatory diseases, like rheumatoid arthritis (RA). A systematic structure-activity relationship study with the goal of optimizing lipophilicity successfully yielded two lead compounds, and .
View Article and Find Full Text PDFVoltage-gated sodium channel Na1.7 is a genetically validated target for pain. Identification of Na1.
View Article and Find Full Text PDFThe present work was to study induction of cytochrome P450 (CYP)3A and CYP2H1 gene by reverse transcriptase polymerase chain reaction (RT-PCR) and quantitative RTPCR in Bantam, Bantamized White Leghorn and White Leghorn chicks. Out of 18 chicks total 3 from each group (Bantam, Bantamized White Leghorn and White Leghorn) were treated intraperitoneal with phenobarbital at the dose rate of 12 mg/100 g (body weight) while the control group was treated with the saline. Total RNA was extracted from the liver samples using Tri Reagent based method.
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