Whole-Genome Sequence of Geobacillus thermoleovorans ARTRW1, Isolated from Armutlu Geothermal Spring, Turkey.

The thermophilic microorganism ARTRW1 was isolated from water samples collected in the Armutlu hot spring in Turkey. Here, the whole-genome sequence and its annotations are reported.

Matrix metalloproteinase gene expressions might be oxidative stress targets in gastric cancer cell lines.

Objective: Oxidative stress is linked to increased risk of gastric cancer and matrix metalloproteinases (MMPs) are important in the invasion and metastasis of gastric cancer. We aimed to analyze the effect of the accumulation of oxidative stress in the gastric cancer MKN-45 and 23132/87 cells following hydrogen peroxide (H2O2) exposure on the expression patterns of MMP-1, MMP-3, MMP-7, MMP-9, MMP-10, MMP-11, MMP-12, MMP-14, MMP-15, MMP-17, MMP-23, MMP-28, and β-catenin genes.

Methods: The mRNA transcripts in the cells were determined by RT-PCR.

Silencing of the MMP-3 gene by siRNA transfection in gastric cancer AGS cells.

Background: The gastric epithelium is continuously exposed to toxic reactive oxygen species and matrix metalloproteinases (MMPs) are the enzymes known for their roles in the invasion of tumor cells. Here, we report the suppression of matrix metalloproteinase 3 (MMP-3) in gastric cancer cell line AGS by small interfering RNA (siRNA) transfection and its potential role in gastric cancers.

Methods: Oxidative stress in the cell lines was assessed following H2O2 exposure using an oxidative stress marker, 2,7-dichlorofluorescein diacetate (DCFDA).