Nanotopographic characterization of spotted micro arrays on polyvinyl alcohol films by high-resolution long-range nanoprofiling.

The nanopositioning and nanomeasuring machine was applied for the nanotopographic characterization of polymer micro spot arrays of fluorimetric chemochips. Chemochips are arrays of fluorescence dyes in a hydrogel matrix with different response behaviors of chemical components determination of chemical and physico-chemical properties of analytes by a pattern recognition approach. For the characterization and quality control of the spots, a nanometer resolution is needed over a scan range of several millimeters.

Differentiation of liquid analytes in gel films by permeability-modulated double-layer chemo-chips.

A double-layer chemo-chip for the characterization of liquid analytes by rapid fluorimetric imaging is described. The chemo-chip consists of an array of polymeric micro-spots prepared on a glass slide. Each spot is composed of a thin indicator layer made of PVA doped with an immobilized fluorescence dye and a top layer polymer spot with different permeation properties.