Publications by authors named "Angelo Bitetti"
The number of annotated long noncoding RNAs (lncRNAs) continues to grow; however, their functional characterization in model organisms has been hampered by the lack of reliable genetic inactivation strategies. While partial or full deletions of lncRNA loci disrupt lncRNA expression, they do not permit the formal association of a phenotype with the encoded transcript. Here, we examined several alternative strategies for generating lncRNA null alleles in zebrafish and found that they often resulted in unpredicted changes to lncRNA expression.
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- Scientists found that a special type of tiny RNA called miRNA can control other RNA messages in the body, but some strong connections can cause the miRNA to get destroyed instead.
- They studied how this works in zebrafish and mice, discovering that a specific RNA can change how these animals behave.
- When they changed the part of the RNA that connects with miRNA in mice, it messed up their movement and learning because the miRNA couldn't do its job properly.
Article Synopsis
- Spermatogenesis is the process where sperm cells are made, and it causes big changes in chromosomes and DNA.
- Researchers found that certain parts of viruses in mice help produce specific long RNA molecules, mostly in sperm cells.
- These viral parts can act like special switches to control gene expression, which can lead to the creation of new genes and adds variety to how sperm cells work.
Super-enhancers (SEs) are key transcriptional drivers of cellular, developmental, and disease states in mammals, yet the conservational and regulatory features of these enhancer elements in nonmammalian vertebrates are unknown. To define SEs in zebrafish and enable sequence and functional comparisons to mouse and human SEs, we used genome-wide histone H3 lysine 27 acetylation (H3K27ac) occupancy as a primary SE delineator. Our study determined the set of SEs in pluripotent state cells and adult zebrafish tissues and revealed both similarities and differences between zebrafish and mammalian SEs.
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