Use of hyaluronidase for pharmacokinetic increase in bioavailability of intracutaneously applied substances.

Endogenous hyaluronidases in the human body have physiological and pathophysiological significance due to their enzymatic activity during the cleavage of hyaluronan. The potential of therapeutic use of testicular and recombinant hyaluronidase has been increasingly acknowledged and developed in recent years. Most relevant in practice is the co-application of hyaluronidase and other active substances in order to enhance their bioavailability within the tissue.

Clinical trial for safety evaluation of hyaluronidase as diffusion enhancing adjuvant for infiltration analgesia of skin with lidocaine.

Background: Infiltration anesthesia of the skin is an analgesic procedure often practiced before minor surgical interventions or punctures. The addition of hyaluronidase is a possible option to improve the effectiveness of the local anesthetic with respect to expansion of effect.

Objective: To validate the safety of intracutaneous application of hyaluronidase as a lidocaine adjuvant.