Publications by authors named "Angelica Osorio-Espinoza"
Histamine H₃ receptors (H₃Rs) modulate the function of the nervous system at the pre- and post-synaptic levels. In this work we aimed to determine whether, as other G protein-coupled receptors (GPCRs), H₃Rs desensitize in response to agonist exposure. By using CHO-K1 cells stably transfected with the human H₃R (hH3R) we show that functional responses (inhibition of forskolin-induced cAMP accumulation in intact cells and stimulation of [(35)S]-GTPγS binding to cell membranes) were markedly reduced after agonist exposure.
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- The study investigates the effects of a mutation (A280V) in the human histamine H₃ receptor, originally linked to Shy-Drager syndrome and migraine risk.
- Researchers compared the wild-type receptor with the A280V mutant using CHO-K1 cells to assess receptor expression, affinity, and functional signaling.
- Findings reveal that the A280V mutation diminishes the receptor's signaling efficacy, which could have implications for understanding related health disorders.
We have studied the effect of histamine H(3) receptor (H(3)R) activation on the depolarization-evoked release of labeled neurotransmitters from slices of the rat olfactory bulb (rOB). The presence of pre-synaptic H(3)Rs was evidenced by the specific binding of the H(3)R ligand N-α-[methyl-(3)H]histamine to membranes from rOB synaptosomes (maximum binding, B(max), 106 ± 19 fmol/mg protein; dissociation constant, K(d), 0.68 ± 0.
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