Alterations of the number and the profile of myenteric neurons of Wistar rats promoted by age.

The objective of this study was to analyze the morpho quantitative behaviour of duodenum myenteric neurons of Wistar rats (Rattus norvegicus), aged 21, 60, 90, 210, 345 and 428 days, using wholemount preparations of the muscular tunica obtained by dissecting the intestinal tunica for neuronal revealing, through the Giemsa non-histochemical and Myosin-V immunohistochemical techniques. The neurons were quantified in 80 microscopic fields (14.832 mm(2)) for each animal and neuronal cell body morphometry was carried out on 100 neurons/rat.