Publications by authors named "Angeles E Espino-Saldana"
Article Synopsis
- * In adult cerebellum, particularly in Bergmann glia, glutamatergic signaling is essential for motor coordination, but the origins of calcium activity in these cells during development have not been thoroughly studied.
- * Research using transgenic zebrafish revealed that radial glia undergo significant morphological changes shortly after fertilization, with calcium activity becoming more prominent and dynamic as the cells mature, indicating important links between cell structure and function in brain development.
Article Synopsis
- * The study focused on the role of a specific amino acid sequence, LCI, located near the selectivity filter GYG in HCN channels, which is not present in standard potassium channels.
- * Using experiments on frog oocytes, researchers discovered that the LCI sequence influences how HCN2 currents operate, particularly affecting ion permeability and gating, with the isoleucine (I) residue being especially important.
Article Synopsis
- Researchers explored the four mctp genes in zebrafish (mctp1a, mctp1b, mctp2a, and mctp2b), examining their structure, expression during development, and role in adult tissues.
- They found that these genes are primarily active in the nervous and muscular systems, with different expression patterns starting from early development.
- Knocking down mctp2b using CRISPR/Cas9 led to impaired embryonic development, indicating its crucial role during early stages.
Article Synopsis
- Danio rerio (zebrafish) is an effective model organism for studying genetics and human diseases, particularly in neuroscience, due to its unique advantages like rapid development and shared neurobiological traits with humans.
- * Recent studies using CRISPR technology on zebrafish have significantly advanced our understanding of neuronal functions and brain disorders, showcasing the potential for this method in neurology.
- * Despite the progress made with CRISPR technologies in zebrafish, further interdisciplinary collaboration is essential to uncover the causes and treatments for various brain diseases.
Phosphatidylinositol 4,5-bisphosphate, cholesterol, and fatty acids modulate the calcium-activated chloride channel TMEM16A (ANO1).
Biochim Biophys Acta Mol Cell Biol Lipids
March 2018
The TMEM16A-mediated Ca-activated Cl current drives several important physiological functions. Membrane lipids regulate ion channels and transporters but their influence on members of the TMEM16 family is poorly understood. Here we have studied the regulation of TMEM16A by phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), cholesterol, and fatty acids using patch clamp, biochemistry and fluorescence microscopy.
View Article and Find Full Text PDFHere, we describe an outward rectifying current in Xenopus tropicalis oocytes that we have called xtClC-or. The current has two components; the major component is voltage activated and independent of intracellular or extracellular Ca(2+), whereas the second is a smaller component that is Ca(2+) dependent. The properties of the Ca(2+)-independent current, such as voltage dependence and outward rectification, resemble those of ClC anion channels/transporters.
View Article and Find Full Text PDFGamma-aminobutyric acid (GABA)ρ receptors are selectively targeted to the axon terminals of the retinal bipolar neurons. The traffic of a green fluorescent protein-tagged GABAρ2 was examined in retinal bipolar neurons and cerebellar astrocytes. In bipolar neurons, time-lapse laser confocal microscopy revealed that the fluorescence emitted by GABAρ2-green fluorescent protein accumulates first, in clusters, in the soma and is then distributed along the axon in at least two populations: one that remains relatively immobile and a second population of smaller clusters that moved constantly to and from the axon end.
View Article and Find Full Text PDFHexavalent chromium removal in vitro and from industrial wastes, using chromate-resistant strains of filamentous fungi indigenous to contaminated wastes.
Can J Microbiol
September 2006
Two chromate-resistant filamentous fungi, strains H13 and Ed8, were selected from seven independent fungal isolates indigenous to Cr(VI)-contaminated soil because of their ability to decrease hexavalent chromium levels in the growth medium. Morphophysiological studies identified strain H13 as a Penicillium sp. isolate and Ed8 as an Aspergillus sp.
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