Publications by authors named "Angela de Haan"
Article Synopsis
- Multidrug-resistant organisms (MDRO) are a major public health concern, and understanding their genomic characteristics is vital for monitoring and controlling infections.
- This study focuses on the use of long-read whole-genome sequencing (WGS) to analyze the molecular profiles of 356 MDRO isolates, including various bacteria such as Klebsiella pneumoniae and methicillin-resistant Staphylococcus aureus (MRSA).
- Results showed that long-read sequencing provides comparable molecular data to short-read sequencing, with high consistency in multi-locus sequence typing (wgMLST) profiles, though some differences were noted in Pseudomonas aeruginosa.
Article Synopsis
- A significant rise in multidrug-resistant microorganisms (MDRO) was noted following the transfer of Ukrainian patients to hospitals, with various resistant strains identified, including carbapenem-resistant bacteria.
- Testing for antimicrobial susceptibility showed low efficacy rates for several last-resort antibiotics, highlighting severe resistance issues among these pathogens.
- Factors influencing cefiderocol susceptibility results include testing methods and standards used, making understanding resistance patterns crucial for treating patients suspected of Gram-negative infections with recent hospitalizations in Ukraine.
Article Synopsis
- There is a rising rate of fatty liver disease in the western world, primarily due to steatosis, which can be caused by certain foods, excessive alcohol or fat intake, and some medications.
- Advances in understanding how chemicals cause steatosis have led to the creation of adverse outcome pathways (AOPs), outlining how chemical exposure results in liver fat buildup.
- In a study using zebrafish embryos, three pesticides were tested for their impact on liver triglyceride accumulation, showing that zebrafish are effective for evaluating these effects and confirming chemical-induced steatosis, with findings supporting that similar and different acting chemicals can add up in their effects.
BackgroundThe war in Ukraine led to migration of Ukrainian people. Early 2022, several European national surveillance systems detected multidrug-resistant (MDR) bacteria related to Ukrainian patients.AimTo investigate the genomic epidemiology of New Delhi metallo-β-lactamase (NDM)-producing from Ukrainian patients among European countries.
View Article and Find Full Text PDFDuring 2015-2022, a genetic cluster of OXA-48-producing uropathogenic Escherichia coli sequence type 127 spread throughout the Netherlands. The 20 isolates we investigated originated mainly from urine, belonged to Clermont phylotype B2, and carried 18 genes encoding putative uropathogenicity factors. The isolates were susceptible to first-choice antimicrobial drugs for urinary tract infections.
View Article and Find Full Text PDFBackground: Although the Netherlands is a country with a low endemic level, methicillin-resistant Staphylococcus aureus (MRSA) poses a significant health care problem. Therefore, high coverage national MRSA surveillance has been in place since 1989. To monitor possible changes in the type-distribution and emergence of resistance and virulence, MRSA isolates are molecularly characterized.
View Article and Find Full Text PDFSince March 2022, there has been an emergence of multidrug-resistant organisms (MDRO) in the Netherlands in patients originating from Ukraine (58 patients, 75 isolates). For about half of these patients, recent hospitalisation in Ukraine was reported. Genomic surveillance revealed that the majority of the MDRO represent globally spread epidemic lineages and that 60% contain New Delhi metallo-β-lactamase (NDM) genes.
View Article and Find Full Text PDFStaphylococcus argenteus is a recently described member of the Staphylococcus aureus complex (SAC) and is associated with human disease. The frequency and intensity of infections caused by are similar to those of Staphylococcus aureus. can harbor antibiotic resistance genes and a variety of virulence factors analogous to methicillin-resistant S.
View Article and Find Full Text PDFArticle Synopsis
- * A study involving 22 Dutch laboratories analyzed 72 pairs of colistin-resistant (COLR-EK) and colistin-susceptible (COLS-EK) bacterial isolates, revealing that 21% of COLR-EK patients had previously received colistin treatment.
- * While colistin resistance remains relatively rare in the Netherlands, some isolates showed genetic mutations linked to resistance, highlighting a need for ongoing monitoring and testing practices.
Background: Carbapenemases produced by Enterobacterales are often encoded by genes on transferable plasmids and represent a major healthcare problem, especially if the plasmids contain additional antibiotic resistance genes. As part of Dutch national surveillance, 50 medical microbiological laboratories submit their Enterobacterales isolates suspected of carbapenemase production to the National Institute for Public Health and the Environment for characterization. All isolates for which carbapenemase production is confirmed are subjected to next-generation sequencing.
View Article and Find Full Text PDFCarbapenem-hydrolysing enzymes belonging to the OXA-48-like group are encoded by -like alleles and are abundant among in the Netherlands. Therefore, the objective here was to investigate the characteristics, gene content and diversity of the -like carrying plasmids and chromosomes of and collected in the Dutch national surveillance from 2014 to 2019 in comparison with genome sequences from 29 countries. A combination of short-read genome sequencing with long-read sequencing enabled the reconstruction of 47 and 132 complete -like plasmids for and , respectively.
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- Carbapenemase-producing Klebsiella pneumoniae has become a significant hospital-acquired pathogen, leading to increased illness and death, necessitating close monitoring of its spread and genetic diversity among patients.
- The study aimed to analyze and track the genetic makeup of K. pneumoniae strains with a specific beta-lactamase gene (bla) in the Netherlands and former Dutch Caribbean islands from 2014 to 2019 using advanced sequencing techniques.
- Findings revealed distinct genetic clusters and variations in plasmids, indicating different antibiotic resistance and virulence characteristics, particularly highlighting the transmission of a specific strain (KpnCluster-019 bla) between regions.
Objectives: To investigate prolonged carriage of MRSA in adults from the general population living in a livestock-dense area, using WGS.
Methods: A cross-sectional study during 2014-15 among 2492 adults without professional livestock contact identified 14 (0.6%) nasal MRSA carriers, 10 of which carried livestock-associated (LA)-MRSA of multiple-locus variable-number tandem repeat analysis (MLVA) complex (MC) 398.
Objectives: In 2005, 39% of pigs and 81% of the slaughter batches at Dutch slaughterhouses were MRSA positive. The objective of the present study was to investigate whether the 50% reduction of antimicrobial usage in finishing pigs in 2014 compared with 2009 in the Netherlands has led to a lower MRSA prevalence among Dutch slaughter pigs.
Methods: Nasal swabs from eight slaughter batches of on average 10 animals at seven slaughterhouses were taken and cultured using method 1, which was used in 2005, and method 2, using high-salt pre-enrichment.
A new phenotypic test, called the Carbapenem Inactivation Method (CIM), was developed to detect carbapenemase activity in Gram-negative rods within eight hours. This method showed high concordance with results obtained by PCR to detect genes coding for the carbapenemases KPC, NDM, OXA-48, VIM, IMP and OXA-23. It allows reliable detection of carbapenemase activity encoded by various genes in species of Enterobacteriaceae (e.
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