Simultaneous overexpression of human E5NT and ENTPD1 protects porcine endothelial cells against HO-induced oxidative stress and cytotoxicity in vitro.

Ischemia-reperfusion injury (IRI) and oxidative stress still limit the survival of cells and organs in xenotransplantation models. Ectonucleotidases play an important role in inflammation and IRI in transplantation settings. We tested the potential protective effects derived by the co-expression of the two main vascular ectonucleotidases, ecto-5'-nucleotidase (E5NT) and ecto nucleoside triphosphate diphosphohydrolase 1 (ENTPD1), in an in vitro model of HO-induced oxidative stress and cytotoxicity.