Polymer dots and glassy organic dots using dibenzodipyridophenazine dyes as water-dispersible TADF probes for cellular imaging.

Fluorescence imaging of living cells is key to better understanding cellular morphology and biological processes. Water-dispersible nanoparticles exhibiting thermally activated delayed fluorescence (TADF) have recently emerged as useful probes for time-resolved fluorescence imaging (TRFI), circumventing interference from biological autofluorescence. Many existing approaches, however, require TADF dyes with specific structural features, precluding many high-performance TADF materials from being used in this application.