In vitro exposure to carbon dioxide induces oxidative stress in human peritoneal mesothelial cells.

Background: The objective of this study was to verify whether in vitro exposure of human peritoneal mesothelial cells to carbon dioxide (CO(2)) influences the levels of 8-isoprostaglandin F(2alpha) (8-iso-PGF(2alpha)), a marker of oxidative stress.

Methods: Mesothelial cells were exposed to either: (i). 100% CO(2) for 4 h; (ii).

The effect of intra-abdominal pressure on the generation of 8-iso prostaglandin F2alpha during laparoscopy in rabbits.

Background: Carbon dioxide pneumoperitoneum induces peritoneal oxidative stress. The aim of this study was to verify the effect of intra-abdominal pressure on oxidative stress in the peritoneum and on post-operative adhesion formation.

Methods: Forty-one rabbits underwent laparoscopic surgery: either gasless, or with CO(2)-pneumoperitoneum at pressures of 5, 10 or 15 mmHg.

Comparison of peritoneal oxidative stress during laparoscopy and laparotomy.

Study Objective: To identify oxidative stress in peritoneum during laparoscopic and open surgery by measuring products of lipid peroxidation, and to determine whether surgical approach influences the type of oxidative metabolite synthesized.

Design: Retrospective analysis (Canadian Task Force classification II-2).

Setting: University-affiliated hospital.