Cell-Free Screening, Production and Animal Testing of a STI-Related Chlamydial Major Outer Membrane Protein Supported in Nanolipoproteins.

Background: Vaccine development against Chlamydia, a prevalent sexually transmitted infection (STI), is imperative due to its global public health impact. However, significant challenges arise in the production of effective subunit vaccines based on recombinant protein antigens, particularly with membrane proteins like the Major Outer Membrane Protein (MOMP).

Methods: Cell-free protein synthesis (CFPS) technology is an attractive approach to address these challenges as a method of high-throughput membrane protein and protein complex production coupled with nanolipoprotein particles (NLPs).

Strengthening the Voices of Hispanic/ Immigrants Managing Chronic Disease: A Mixed Methods Approach to Understanding Perspectives of Health.

Individuals who migrate from their home country face a variety of challenges while adapting to the culture in the United States. Immigrant communities are at a significantly higher risk for poor health outcomes; therefore, assessing healthcare treatment for diverse and resilient immigrant populations, including Hispanic/ communities, is crucial to preserving their health, culture, and spirit. A paucity of literature exists surrounding perceptions of well-being in immigrant, Hispanic/ adults managing chronic diseases.

Attorneys' Questions About Time in Criminal Cases of Alleged Child Sexual Abuse.

In cases of alleged child sexual abuse, information about the timing of events is often needed. However, published developmental laboratory research has demonstrated that children struggle to provide accurate and reliable testimony about time and there is currently a lack of field research examining how attorneys actually question child witnesses about time in court. The current study analyzed 130 trial transcripts from cases of alleged child sexual abuse containing a child witness between the ages of 5-17 years old to determine the frequency, style, and content of attorneys' questions and child responses about time.

Lawyers rapport building practices with child witnesses.

Background: Existing literature on rapport building in forensic interviews with children has primarily focused on police or social workers (Collins et al., 2002); overlooking the lawyer-child relationship.

Objective: The present study was a novel exploration of the rapport building process between lawyers and child witnesses during the interview stage of a criminal proceeding.

Development, validation, and psychometric analysis of Foot and Ankle Flexibility Index (FAFI).

Aim: To develop a new tool for identifying joint hypermobility of the paediatric foot and ankle, based on a dichotomous scoring system utilising the Lower Limb Assessment Score (LLAS), to separate the foot and ankle items.

Material And Methods: A total of 205 children, aged between 5 and 10 years, participated in a cross-sectional study. The new tool Foot and Ankle Flexibility Index (FAFI) was predicated upon the last 7 items of LLAS, which are specific to the foot and ankle.

Longitudinal associations between lie evaluations and frequency: The moderating role of age.

While previous studies have demonstrated correlations between children and adolescents' evaluations of lies and lie-telling behaviors, the temporal order of these associations over time and changes across this developmental period remain unexamined. The current study examined longitudinal associations among children and adolescents' (N = 1128; M = 11.54, SD = 1.

The resting calcaneal stance position (RCSP): an old dog, with new tricks.

The objective of this study was to establish the accuracy of the resting calcaneal stance position (RCSP) for the assessment of flat foot (FF) in children, aligned to the validity of the foot posture index (FPI). The RCSP cut-off point was explored, in context of both FF prevalence and the relationship between FF and body weight. A total of 205 healthy children, aged 5 to 10 years, participated in a cross-sectional study.

"Locked in a Jail Cell in Your Own Home": Child Maltreatment Investigators' Perspectives of COVID-19's Effects on Maltreated Children.

Children were at a greater risk of adverse experiences, including maltreatment, during the COVID-19 pandemic given the increased stress experienced by families and reduced visibility outside the home. Child maltreatment investigators witnessed the effects of the pandemic on maltreated children and offer valuable insight regarding children's experiences during the pandemic. The objective of this study was to examine child maltreatment investigators' perspectives of the impact of the COVID-19 pandemic on maltreated children and their families in Canada.

Moral evaluations of children's truths and lies in a prosocial context: The role of reputation.

The current investigation examined the influence of a child's reputation on 7- to 12-year-olds' (Study 1; N = 146) and parents' (Study 2; N = 198) moral evaluations of the child's blunt truths (i.e., truths told despite possible hurt feelings) and prosocial lies (i.

Encouraging honesty: Developmental differences in the influence of honesty promotion techniques.

Given the value placed on honesty and the negative consequences of lying, encouraging children's truth-telling is important. The present investigation assessed honesty promotion techniques for encouraging 3-8-year-old Canadian children's (Study 1: = 301, 54% female; Study 2: = 229, 50% female from predominantly White middle-class samples) disclosure of a transgression and whether they varied by age. Study 1 examined promising to tell the truth, inducing self-awareness, and the combination of both promising and self-awareness.

Describing Coercion in the Courtroom: The Influence of Language and Maltreatment Severity on Jurors' Perceptions of Child Witnesses.

Regardless of compliance to coercion by an alleged perpetrator, child maltreatment is abuse in any form. However, the extent to which coercion is described as an obligation (mandatory compliance) or permission (optional compliance) is legally relevant. The present investigation examined how attorneys question children about coercion and how children describe coercion in courtroom investigations of alleged child sexual abuse, and whether such language influences jurors' perceptions of children's testimony.

Avoidant Parent-Child Communication About COVID-19: A Longitudinal Investigation of Associations with Youth Adjustment Across the First 6 Months of the Pandemic.

In the aftermath of discrete disasters, how families discuss the event has been linked with child well-being. There is less understanding, however, of how family communication affects adjustment to a protracted and ongoing public health crisis such as the COVID-19 pandemic. The present research leveraged a large longitudinal sample of families (N = 1884) across the United States and Canada to investigate factors that predicted family communication styles (active versus avoidant communication) about the COVID-19 pandemic and examined the longitudinal sequelae of mental health outcomes for youth associated with different family communication styles.

A taxonomy of groomer profiles: Comparisons of in-person, online, and mixed groomers through the examination of Canadian judicial decisions.

Background: Sexual grooming in cases of child sexual abuse (CSA) has captured the attention of researchers over the past decades. While early research focused on offenders who groomed children in person, our knowledge of online groomers has begun to increase. However, there has not been a concomitant increase in understanding of groomers who use both in-person and online grooming strategies (i.

A Latent Profile Analysis of Lie-Telling to Parents and Friends during Adolescence.

Adolescence has been suggested to be a time of heightened lie-telling. The current study used a latent profile analysis to examine unique patterns of lie-telling for lies told to parents and friends during adolescence as well as whether adjustment indicators (relationship quality, depressive symptoms, social anxiety, externalizing problems) could be used to predict group membership. These patterns were examined among 828 10- to 16- year-olds (M = 12.

Graphene as a rational interface for enhanced adsorption of microcystin-LR from water.

Cyanotoxins such as microcystin-LR (MC-LR) represent a global environmental threat to ecosystems and drinking water supplies. The study investigated the direct use of graphene as a rational interface for removal of MC-LR via interactions with the aromatic ring of the ADDA chain of MC-LR and the sp hybridized carbon network of graphene. Intra-particle diffusion model fit indicated the high mesoporosity of graphene provided significant enhancements to both adsorption capacities and kinetics when benchmarked against microporous granular activated carbon (GAC).

Exploring the distribution of risk factors for drop-out from Ponseti treatment for clubfoot across Bangladesh using geospatial cluster analysis.

Clubfoot is a congenital anomaly affecting 1/1,000 live births. Ponseti casting is an effective and affordable treatment. About 75% of affected children have access to Ponseti treatment in Bangladesh, but 20% are at risk of drop-out.

Child Witnesses Productively Respond to "How" Questions About Evaluations but Struggle With Other "How" Questions.

Child interviewers are often advised to avoid asking "How" questions, particularly with young children. However, children tend to answer "How" evaluative questions productively (e.g.

Emergency examination authorities in Queensland, Australia.

Objective: In Queensland, where a person experiences a major disturbance in their mental capacity, and is at risk of serious harm to self and others, an emergency examination authority (EEA) authorises Queensland Police Service (QPS) and Queensland Ambulance Service (QAS) to detain and transport the person to an ED. In the ED, further detention for up to 12 h is authorised to allow the examination to be completed. Little published information describes these critical patient encounters.

Longitudinal associations between impulsivity and lie-telling in childhood and adolescence.

Lie-telling and impulsivity levels peak during late childhood to early adolescence and have been suggested to be related. Heightened impulsivity may lead adolescents to lie in favor of short-term benefits without consideration for the potential consequences of deception. The present study assessed longitudinal relations between self-reported impulsivity and lie-telling frequency.

Maltreated and non-maltreated children's truthful and dishonest reports: Linguistic and syntactic differences.

Introduction: Adults are typically poor judges of the veracity of statements, requiring the need for alternative methods for detecting lies. One alternative method to human lie-detectors is using computer-based linguistic analysis which may present a more reliable method for detecting dishonesty. Moreover, while previous research has examined linguistic differences between typically developing children's and adults' truthful and dishonest reports, no study to date has examined whether maltreated children exhibit different linguistic cues to dishonesty.

Immediate effects of acute Mars mission equivalent doses of SEP and GCR radiation on the murine gastrointestinal system-protective effects of curcumin-loaded nanolipoprotein particles (cNLPs).

Introduction: Missions beyond low Earth orbit (LEO) will expose astronauts to ionizing radiation (IR) in the form of solar energetic particles (SEP) and galactic cosmic rays (GCR) including high atomic number and energy (HZE) nuclei. The gastrointestinal (GI) system is documented to be highly radiosensitive with even relatively low dose IR exposures capable of inducing mucosal lesions and disrupting epithelial barrier function. IR is also an established risk factor for colorectal cancer (CRC) with several studies examining long-term GI effects of SEP/GCR exposure using tumor-prone APC mouse models.

Modulation of Radiation Biomarkers in a Randomized Phase II Study of I-MIBG With or Without Radiation Sensitizers for Relapsed or Refractory Neuroblastoma.

Purpose: I-metaiodobenzylguanidine (I-MIBG) has demonstrated efficacy as a single agent in neuroblastoma. Recent trials have focused on I-MIBG combination strategies, though little is known about the effect of putative radiosensitizers on biological markers of radiation exposure.

Methods And Materials: NANT2011-01 evaluated I-MIBG therapy alone (arm A) or in combination with vincristine/irinotecan (arm B) or vorinostat (arm C) for patients with relapsed or refractory neuroblastoma.

Children's foot parameters and basic anthropometry - do arch height and midfoot width change?

Unlabelled: The aims of this study were as follows: (1) to assess how foot posture and morphology assessments change according to body mass index (BMI) status; (2) to determine which body composition parameter (BMI or waist circumference) correlates better with the foot posture index (FPI), arch height index (AHI), and midfoot width (MFW) in children. Foot morphometry (FPI, AHI, and MFW) and body composition (BMI and waist circumference (WC)) were assessed in a cross-sectional study of 575 children (mean age = 7.42 ± 1.

The Impact of the Consistency of Child Witness and Peer Reports on Credibility.

Adults' perceptions of children's disclosures have important implications for the response to that disclosure. Children who experience adult transgressions, such as maltreatment, often choose to disclose this experience to a peer. Thus, peer disclosure recipients may transmit this disclosure to an adult or provide support for the child's own disclosure.

Lawyers' experience questioning children in Canadian court.

Background: International research has explored lawyer-child interactions in court; however, little focus has been spent examining other aspects of lawyers' interactions with children (e.g., interview preparation; building rapport).