Our objective was to determine the effects of chemical structure, amount, and site of infusion of long-chain fatty acids (LCFA) in lactating dairy cows. Six multiparous Holstein cows were used in a 6 × 6 Latin square design with 21-d periods. During d 1 to 14, 250 g/d of LCFA and during d 15 to 21, 500 g/d of LCFA were infused continuously into either the rumen or abomasum.
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