Bacterial biosurfactant increases ex situ biodiesel bioremediation in clayey soil.

The contamination of soils by oily compounds has several environmental impacts, which can be reversed through bioremediation, using biosurfactants as auxiliaries in the biodegradation process. In this study, we aimed to perform ex situ bioremediation of biodiesel-contaminated soil using biosurfactants produced by Bacillus methylotrophicus. A crude biosurfactant was produced in a whey-based culture medium supplemented with nutrients and was later added to biodiesel-contaminated clayey soil.

Effects of homemade biosurfactant from Bacillus methylotrophicus on bioremediation efficiency of a clay soil contaminated with diesel oil.

Despite constant progress in the understanding of the mechanisms related to the effects of biosurfactants in the bioremediation processes of oily residues, the possibility of antagonist effects on microbial growth and the production in situ of these compounds must be elucidated. The aims of this work were a) to evaluate the effects of the addition of a homemade biosurfactant of Bacillus methylotrophicus on the microbial count in soil in order to determine the possibility of inhibitory effects, and b) to accomplish biostimulation using media prepared with whey and bioaugmentation with B. methylotrophicus, analyzing the effects on the bioremediation of diesel oil and evidencing the in situ production of biosurfactants through effects on surface tension.

Biosurfactants during in situ bioremediation: factors that influence the production and challenges in evalution.

Research on the influence of biosurfactants on the efficiency of in situ bioremediation of contaminated soil is continuously growing. Despite the constant progress in understanding the mechanisms involved in the effects of biosurfactants, there are still many factors that are not sufficiently elucidated. There is a lack of research on autochthonous or exogenous microbial metabolism when biostimulation or bioaugmentation is carried out to produce biosurfactants at contaminated sites.