A metabolomics strategy for detecting protein-metabolite interactions to identify natural nuclear receptor ligands.

Nuclear receptors are ligand-regulated transcription factors that control a range of (patho)physiological processes. Lipids are the most common type of nuclear receptor ligands. Here, we utilize a metabolomics approach that detects protein-metabolite interactions to identify endogenous peroxisome proliferator-activated receptor (PPAR) ligands from tissue and cell extracts.

Regional, cellular, and subcellular localization of RGS10 in rodent brain.

The regulator of G protein signaling type 10 (RGS10) modulates Galphai/o signaling by means of its GTPase accelerating activity and is abundantly expressed in brain and in immune tissues. To elucidate RGS10 function in the nervous system, we mapped RGS10 protein in rat and mouse brain using light microscopic (LM) and electron microscopic (EM) immunohistochemical techniques. The LM showed that RGS10-like immunoreactivity (LIR) labels all cellular subcompartments of neurons and microglia, including their nuclei.