Packaged delivery of CRISPR-Cas9 ribonucleoproteins accelerates genome editing.

Effective genome editing requires a sufficient dose of CRISPR-Cas9 ribonucleoproteins (RNPs) to enter the target cell while minimizing immune responses, off-target editing and cytotoxicity. Clinical use of Cas9 RNPs currently entails electroporation into cells , but no systematic comparison of this method to packaged RNP delivery has been made. Here we compared two delivery strategies, electroporation and enveloped delivery vehicles (EDVs), to investigate the Cas9 dosage requirements for genome editing.

Genetic parameters for milk yield and reproductive traits in Honduran Holstein cattle.

The aim of this study was to estimate the (co)variance components and genetic parameters for milk yield adjusted to 305d (MY305), calving-to-conception interval (CCI), number of services per conception (NSC) and calving interval (CI) of Honduran Holstein cows, by fitting a bivariate animal model using Maximum Restricted Likelihood procedures. Model included the fixed effects of calving number, the contemporary calving group (farm-season-year of calving and the cow age as covariate). The estimated means and standard deviations for MY, CCI, NSC and CI were, 5098.

Design rules for efficient endosomal escape.

The inefficient translocation of proteins across biological membranes limits their application as therapeutic compounds and research tools. In most cases, translocation involves two steps: uptake into the endocytic pathway and endosomal escape. Certain charged or amphiphilic molecules promote protein uptake but few enable efficient endosomal escape.

[Differential diagnosis of cemento-osseous dysplasia of the maxilla. A case report].

Cemento-osseous dysplasia is a benign lesion which affects the jaw bones. It is a frequent incidental finding but no aggressive therapy is necessary. However, it may be confused with more aggressive entities, such as ossifying and cementifying fibroma, which do require treatment.

Detection of subsp. Assisted by Micro-Raman Spectroscopy under Laboratory Conditions.

subsp. () is a quarantine-worthy pest in México. The implementation and validation of new technologies is necessary to reduce the time for bacterial detection in laboratory conditions and Raman spectroscopy is an ambitious technology that has all of the features needed to characterize and identify bacteria.

[Inflammatory myofibroblastic tumor of the breast: A rare entity].

Also known as inflammatory pseudotumor, inflammatory myofibroblastic tumor is now considered a true low-grade neoplasm. Although the lung is the most common site, it has been described in many other locations, including the breast; the first report of breast involvement was by Pettinato et al. in 1988.

δ-Aminolevulinate synthase is required for apical transcellular barrier formation in the skin of the Drosophila larva.

Animals construct a layered skin to prevent dehydration and pathogen entrance. The barrier function of the skin relies on the extensive cross-linking of specialised components. In insects, for instance, epidermal cells produce an apical extracellular cuticle that consists of a network of proteins, chitin and lipids.

EEG feature selection using mutual information and support vector machine: A comparative analysis.

The large number of methods for EEG feature extraction demands a good choice for EEG features for every task. This paper compares three subsets of features obtained by tracks extraction method, wavelet transform and fractional Fourier transform. Particularly, we compare the performance of each subset in classification tasks using support vector machines and then we select possible combination of features by feature selection methods based on forward-backward procedure and mutual information as relevance criteria.

New approach in features extraction for EEG signal detection.

This paper describes a new approach in features extraction using time-frequency distributions (TFDs) for detecting epileptic seizures to identify abnormalities in electroencephalogram (EEG). Particularly, the method extracts features using the Smoothed Pseudo Wigner-Ville distribution combined with the McAulay-Quatieri sinusoidal model and identifies abnormal neural discharges. We propose a new feature based on the length of the track that, combined with energy and frequency features, allows to isolate a continuous energy trace from another oscillations when an epileptic seizure is beginning.