Challenges in estimating ancestral state reconstructions: the evolution of migration in Sylvia warblers as a study case.

Our current understanding of how species have evolved is mainly based on comparative phylogenetic methods, which use phylogenies to infer the evolution of traits. The development of ancestral state reconstruction (ASR) methods has provided the tools to reconstruct trait evolution, which are widely used in fields like evolutionary biology, macroecology and paleontology. As there are different elements involved in those analyses, with different levels of uncertainty (i.