Computer analysis of histopathological images for tumor grading. 2.

Objective: We have upgraded our own original color filtration pixel-by-pixel (CFPP) method (Klonowski et al 2018a Physiol. Meas. 39 034002) to enable not only automatic and rapid assessment of the proliferation index of a tumor or neoplasm but also quick automatic location of hot-spots (regions of interest, ROIs) in immunohistochemically stained microscopic images of neoplasms and tumors.